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AB277415

Human Superoxide Dismutase 3/EC-SOD ELISA Kit

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(5 Publications)

Human Superoxide Dismutase 3/EC-SOD ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Superoxide Dismutase 3/EC-SOD in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

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ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB277415)
  • ELISA

Supplier Data

ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB277415)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.65 ng/mL

Range

0.61 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human Superoxide Dismutase 3/EC-SOD ELISA Kit (ab277415) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Superoxide Dismutase 3/EC-SOD in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Superoxide Dismutase 3/EC-SOD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Superoxide Dismutase 3/EC-SOD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Superoxide Dismutase 3/EC-SOD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Superoxide Dismutase 3/EC-SOD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 139 %", "average": "= 118.2" }, { "sample": "Plasma", "range": "116 - 144 %", "average": "= 128.3" }, { "sample": "Cell culture media", "range": "113 - 145 %", "average": "= 129.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277415-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human Superoxide Dismutase 3/EC-SOD standard protein (Lyophilized)", "number":"AB277415-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277415-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human SOD3 coated Microplate", "number":"AB277415-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277415-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277415-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Superoxide Dismutase 3/EC-SOD Antibody", "number":"AB277415-CMP09", "productcode":"" }, { "size":"2 x 30 mL", "name":"Assay Diluent C", "number":"AB277415-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277415-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277415-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.
Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
See full target information Extracellular superoxide dismutase [Cu-Zn]
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2022:6100176 PubMed36311891

2022

LINC02389/miR-7-5p Regulated Cisplatin Resistance of Non-Small-Cell Lung Cancer via Promoting Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Ma,Wen Han,Cunying Meng,Xiaohong Tan,Pengfei Liu,Lei Dong

Cytotechnology 74:579-590 PubMed36238269

2022

Salinomycin suppresses T24 cells by regulating KDM1A and the unfolded protein response pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haofeng Yuan,Yiqian Li,Yun Zou,Chongyue Cai,Xiangmin Shi,Yanfeng Su

Antioxidants (Basel, Switzerland) 11: PubMed35740095

2022

SOD3 and IL-18 Predict the First Kidney Disease-Related Hospitalization or Death during the One-Year Follow-Up Period in Patients with End-Stage Renal Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsien Liu,Yu-Hsuan Chen,Chi-Hua Ko,Chia-Wen Kuo,Chih-Ching Yen,Wei Chen,Kowit-Yu Chong,Chuan-Mu Chen

Frontiers in pharmacology 12:756671 PubMed34899311

2021

Bilateral Paraventricular Nucleus Upregulation of Extracellular Superoxide Dismutase Decreases Blood Pressure by Regulation of the NLRP3 and Neurotransmitters in Salt-Induced Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Su,Xiao-Jing Yu,Xiao-Min Wang,Hong-Bao Li,Ying Li,Juan Bai,Jie Qi,Nianping Zhang,Kai-Li Liu,Yan Zhang,Guo-Qing Zhu,Yu-Ming Kang

Molecular medicine reports 24: PubMed34651666

2021

FABP4 knockdown suppresses inflammation, apoptosis and extracellular matrix degradation in IL-1β-induced chondrocytes by activating PPARγ to regulate the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huajie Mao,Bin Han,Hao Li,Yiqing Tao,Weigang Wu
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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