JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283972

Human Superoxide Dismutase 4/CCS ELISA kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Superoxide Dismutase 4/CCS ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Superoxide Dismutase 4/CCS in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Copper chaperone for superoxide dismutase, Superoxide dismutase copper chaperone, CCS

1 Images
Sandwich ELISA - Human Superoxide Dismutase 4/CCS ELISA kit (AB283972)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 4/CCS ELISA kit (AB283972)

Example of Human Superoxide Dismutase 4/CCS standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Plasma, Serum, Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

> 0.12 ng/mL

Range

0.313 - 20 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human CCS ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab283972) is designed for detection of CCS in human plasma, serum, saliva, cell lysate, and tissue samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CCS in approximately 4 hours.

A polyclonal antibody specific for human CCS has been pre-coated onto a 96- well microplate with removable strips. CCS in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CCS, which is recognized by a streptavidinperoxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283972-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283972-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283972-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"25X Biotinylated Human Superoxide Dismutase 4/CCS Antibody", "number":"AB283972-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283972-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283972-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Superoxide Dismutase 4/CCS Microplate", "number":"AB283972-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB283972-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283972-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283972-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Superoxide Dismutase 4/CCS Standard", "number":"AB283972-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 4 also known as CCS or Copper Chaperone for SOD1 plays a mechanical role as a copper chaperone for the antioxidant enzyme SOD1 (Superoxide Dismutase 1). With a molecular mass of approximately 30 kDa CCS assists in inserting copper into SOD1 activating it for its enzymatic function. CCS is expressed in various tissues including the brain liver and muscle facilitating its role wherever SOD1 activity is needed for protection against oxidative damage.
Biological function summary

CCS contributes significantly to oxidative stress response by catalyzing the dismutation of superoxide radicals into less reactive molecular oxygen and hydrogen peroxide. This protein does not function independently but is part of a complex system involving SOD1 to mitigate oxidative damage within cells. CCS ensures SOD1 acquires the necessary copper which is essential for its dismutase activity hence playing a critical role in maintaining cellular health.

Pathways

The copper delivery to SOD1 by CCS integrates into the broader antioxidant defense pathway. The copper chaperones like CCS link to pathways involving cellular copper homeostasis and oxidative stress management. The protein SOD1 which relies on CCS for copper addition also aligns with the apoptosis and neuroprotective pathways highlighting a shared role in cellular oxidation states control and maintaining neuronal integrity.

CCS associates with conditions such as Amyotrophic Lateral Sclerosis (ALS) and certain neurodegenerative disorders. These conditions relate to the malfunction of the antioxidant defense often involving misfolded SOD1 due to improper copper loading. The impairment in CCS’s role affects not just SOD1 but potentially other copper-dependent proteins exacerbating stress conditions and cellular vulnerability that characterizes these diseases.
style
left-column

Product protocols

style
left-column

Target data

Delivers copper to copper zinc superoxide dismutase (SOD1).
See full target information CCS
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB309312

Human Superoxide Dismutase 1 ELISA Kit

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB309312)

  • 0
  • 0
Assay Kits

AB316899

Human Superoxide Dismutase 3/EC-SOD ELISA Kit

SimpleStep

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB65354

Superoxide Dismutase Activity Assay Kit (Colorimetric)

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com