AB239431

Human Surfactant protein D / SP-D ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human Surfactant protein D / SP-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Surfactant protein D / SP-D with a sensitivity of 4.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

COLEC7, PSPD, SFTP4, SFTPD, Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D

5 Images

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

sELISA

Unknown

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Serum from ten individual healthy human male donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 15.80 ng/mL with a range of 6.86 - 23.16 ng/mL.

sELISA

Unknown

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 12.48 ng/mL with a range of 6.76 - 23.87 ng/mL.

sELISA

Unknown

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Interpolated concentrations of native SP-D in human serum and plasma samples. The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 4580.93 pg/mL in serum, 3466.55 pg/mL in plasma (citrate) and 4166.48 pg/mL in plasma (EDTA), and 4242.64 pg/mL in plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Human SP-D Standard Curve Comparison. Standard Curve comparison between Human SP-D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Example of human SP-D standard curve in Sample Diluent NS.

The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.29 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Surfactant protein D / SP-D ELISA Kit (ab239431) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Surfactant protein D / SP-D protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Surfactant protein D / SP-D with 4.29 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 105 %", "average": "= 99" }, { "sample": "Serum", "range": "89 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "86 - 89 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "81 - 90 %", "average": "= 86" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.

Biological function summary

SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.

Pathways

Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.

SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.

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Product protocols

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Target data

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

See full target information SFTPD

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:4749 PubMed36959289

2023

Pneumoproteins and biomarkers of inflammation and coagulation do not predict rapid lung function decline in people living with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

David M MacDonald,Sarah Samorodnitsky,Chris H Wendt,Jason V Baker,Gary Collins,Monica Kruk,Eric F Lock,Roger Paredes,Selvamuthu Poongulali,Danielle O Weise,Alan Winston,Robin Wood,Ken M Kunisaki

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 115:134-141 PubMed34843955

2021

Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Li,Qian Wu,Zhonghua Qin,Xinwei Hou,Limin Zhang,Jin Guo,Yajie Li,Fangfei Yang,Yan Zhang,Qi Wu,Li Li,Huaiyong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Surfactant protein D / SP-D ELISA Kit

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (AB239431)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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