Human Surfactant protein D / SP-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Surfactant protein D / SP-D with a sensitivity of 4.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Scientific reports 13:47492023Pneumoproteins and biomarkers of inflammation and coagulation do not predict rapid lung function decline in people living with HIV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid M MacDonald,Sarah Samorodnitsky,Chris H Wendt,Jason V Baker,Gary Collins,Monica Kruk,Eric F Lock,Roger Paredes,Selvamuthu Poongulali,Danielle O Weise,Alan Winston,Robin Wood,Ken M KunisakiPubMed 36959289
- International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 115:134-1412021Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Li,Qian Wu,Zhonghua Qin,Xinwei Hou,Limin Zhang,Jin Guo,Yajie Li,Fangfei Yang,Yan Zhang,Qi Wu,Li Li,Huaiyong ChenPubMed 34843955
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.29 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Alternative names
COLEC7, PSPD, SFTP4, SFTPD, Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D
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Human Surfactant protein D / SP-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Surfactant protein D / SP-D with a sensitivity of 4.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.29 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99 | Range 89 - 105 % |
Sample type Serum | Average % = 91 | Range 89 - 96 % |
Sample type EDTA Plasma | Average % = 87 | Range 86 - 89 % |
Sample type Heparin Plasma | Average % = 87 | Range 85 - 90 % |
Sample type Citrate plasma | Average % = 86 | Range 81 - 90 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Surfactant protein D / SP-D ELISA Kit (ab239431) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Surfactant protein D / SP-D protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Surfactant protein D / SP-D with 4.29 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.
- Biological function summary
SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.
- Pathways
Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.
- Associated diseases and disorders
SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.
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5 product images
Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (ab239431)
Human SP-D Standard Curve Comparison. Standard Curve comparison between Human SP-D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (ab239431)
Example of human SP-D standard curve in Sample Diluent NS.
The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (ab239431)
Interpolated concentrations of native SP-D in human serum and plasma samples. The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 4580.93 pg/mL in serum, 3466.55 pg/mL in plasma (citrate) and 4166.48 pg/mL in plasma (EDTA), and 4242.64 pg/mL in plasma (heparin).
Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (ab239431)
Serum from ten individual healthy human male donors were measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 15.80 ng/mL with a range of 6.86 - 23.16 ng/mL.
Sandwich ELISA - Human Surfactant protein D / SP-D ELISA Kit (ab239431)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 12.48 ng/mL with a range of 6.76 - 23.87 ng/mL.
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