JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB119607

Human Survivin ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Survivin ELISA Kit is a Sandwich ELISA for the measurement of Human Survivin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin

1 Images
Sandwich ELISA - Human Survivin ELISA Kit (AB119607)
  • sELISA

Supplier Data

Sandwich ELISA - Human Survivin ELISA Kit (AB119607)

Representative standard curve using ab119607

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 2 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB246533

Human SMAC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SMAC ELISA Kit (AB246533)

  • 0
  • 0
Assay Kits

AB283881

Human ErbB2 / HER2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

  • 0
  • 0
Assay Kits

AB171571

Human p53 ELISA Kit

SimpleStep

ELISA - Human p53 ELISA Kit (AB171571)

  • 0
  • 0
Assay Kits

AB285288

Human TERT ELISA Kit

ELISA - Human TERT ELISA Kit (AB285288)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Human Survivin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Survivin in cell culture supernatants, serum and plasma (heparin, EDTA).

A Survivin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Survivin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Survivin amount of sample captured in plate.

Get better reproducibility in only 90 minutes with Human Survivin ELISA Kit (ab183361) from our SimpleStep ELISA® range.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119607-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119607-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human Survivin antibody", "number":"AB119607-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Survivin antibody Microplate (12 x 8 wells)", "number":"AB119607-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119607-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119607-CMP10", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119607-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119607-CMP08", "productcode":"" }, { "size":"2 x 4 ng", "name":"Lyophilized recombinant Human Survivin standard", "number":"AB119607-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119607-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119607-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119607-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.
Biological function summary

Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.

Pathways

Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.

Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.
style
left-column

Product protocols

style
left-column

Target data

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed : 20627126, PubMed : 21364656, PubMed : 25778398, PubMed : 28218735, PubMed : 9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed : 16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed : 20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed : 20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed : 9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed : 20826784). May play a role in neoplasia (PubMed : 10626797). Inhibitor of CASP3 and CASP7 (PubMed : 21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed : 25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed : 12773388, PubMed : 16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed : 10626797).
See full target information BIRC5
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com