AB183361

Human Survivin ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human Survivin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Survivin with a sensitivity of 7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin

10 Images

ELISA - Human Survivin ELISA Kit (AB183361)

ELISA

Supplier Data

ELISA - Human Survivin ELISA Kit (AB183361)

sELISA

Supplier Data

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Comparison of Survivin concentrations in control and nocodazole treated HeLa cells. HeLa cells were grown in 10F HGDMEM medium for 17 hours in the presence of 200 ng/mL nocodazole or drug's vehicle (DMSO). Survivin concentrations were measured in cell extracts diluted to 125 and 62.5 μg/mL using this kit. Interpolated data values expressed in pg Survivin per µg of cell extract are graphed (mean +/- SD, n=2).

sELISA

Unknown

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Comparison of Survivin concentrations in media and MCF7 cell supernatants. MCF7 cells were grown in 10F HGDMEM medium and Survivin concentrations were measured in undiluted cell culture supernatant (SN) sample and the 10F HGDMEM medium using this kit. Interpolated data values are graphed (mean +/- SD, n=2). Note that no detectable Survivin concentrations in growth medium were observed.

sELISA

Supplier Data

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Survivin concentrations in 10 individual Human serum donors. Two fold diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in pg of Survivin per mL of serum (mean +/- SD, n=3).

sELISA

Unknown

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Example of human Survivin standard curve in 1X Cell Extraction Buffer PTR.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Interpolated concentrations of native Survivin in Jurkat cell extract samples based on a 250 μg/mL extract load.

The concentrations of Survivin were measured in duplicate and interpolated from the Survivin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Survivin concentration was determined to be 4,307.66 pg/mL in Jurkat cell extract.

sELISA

Lab

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Human survivin standard curve comparison data.

Standard curve comparison between human survivin SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Lab

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Interpolated concentration of native Survivin was measured in duplicate at different sample concentrations. Undiluted samples are 1,000 µg/mL Jurkat cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Unknown

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Example of human Survivin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Example of human Survivin standard curve in Sample Diluent 75BP.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Suspension cells, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, Adherent cells

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Survivin ELISA Kit ab183361 is a rapid single-wash 90-min Sandwich ELISA to measure Human Survivin in cell culture supernatant, citrate plasma, serum, suspension cells, tissue extracts, urine. This SimpleStep sensitivity is 7 pg/mL.

How the assay works

Human Survivin SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Survivin ELISA Kit ab183361 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Survivin antibody [EPR2675] - BSA and Azide free (Capture) ab242920
- Anti-Survivin antibody [EPR2880-55] - BSA and Azide free (Detector) ab242920

Survivin is a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and it participates in the organization of the center spindle by associating with polymerized microtubules. Survivin complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Survivin may counteract a default induction of apoptosis in G2/M phase. The acetylated form of Survivin represses STAT3 transactivation of target gene promoters. Survivin may play a role in neoplasia. Survivin is an inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 of Survivin do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Jurkat", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Jurkat", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85.4 - 87.4 %", "average": "= 86.4" }, { "sample": "Serum", "range": "78.5 - 80.5 %", "average": "= 79.5" }, { "sample": "Urine", "range": "89.9 - 92.6 %", "average": "= 91.6" }, { "sample": "Citrate plasma", "range": "94.6 - 102.3 %", "average": "= 99.2" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab183361-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB183361-CMP14" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB183361-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB183361-CMP13" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB183361-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human Survivin Capture Antibody", "number":"AB183361-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human Survivin Detector Antibody", "number":"AB183361-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB183361-CMP05" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB183361-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB183361-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB183361-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB183361-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Survivin Lyophilized Recombinant Protein", "number":"AB183361-CMP09" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB183361-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab183361-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB183361-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB183361-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB183361-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB183361-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB183361-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB183361-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BP", "number":"AB183361-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Survivin Capture Antibody", "number":"AB183361-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB183361-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Survivin Lyophilized Recombinant Protein", "number":"AB183361-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB183361-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Survivin Detector Antibody", "number":"AB183361-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB183361-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.

Biological function summary

Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.

Pathways

Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.

Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.

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Product protocols

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Target data

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed : 20627126, PubMed : 21364656, PubMed : 25778398, PubMed : 28218735, PubMed : 9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed : 16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed : 20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed : 20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed : 9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed : 20826784). May play a role in neoplasia (PubMed : 10626797). Inhibitor of CASP3 and CASP7 (PubMed : 21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed : 25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed : 12773388, PubMed : 16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed : 10626797).

See full target information BIRC5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 69:1833-1840 PubMed32350593

2020

Rapid noninvasive detection of bladder cancer using survivin antibody-conjugated gold nanoparticles (GNPs) based on localized surface plasmon resonance (LSPR).

Applications

Unspecified application

Species

Unspecified reactive species

Mir Hadi Jazayeri,Tayebe Aghaie,Reza Nedaeinia,Mostafa Manian,Hamid Nickho

Cancer genomics & proteomics 15:249-264 PubMed29976630

2018

Effects of Sepantronium Bromide (YM-155) on the Whole Transcriptome of MDA-MB-231 Cells: Highlight on Impaired ATR/ATM Fanconi Anemia DNA Damage Response.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Mazzio,Charles A Lewis,Rashid Elhag,Karam F Soliman

Biomarkers in medicine 11:11-18 PubMed27874286

2016

Identification of bile survivin and carbohydrate antigen 199 in distinguishing cholangiocarcinoma from benign obstructive jaundice.

Applications

sELISA

Species

Human

Yanfeng Liu,Jingxian Sun,Qiangbo Zhang,Bin Jin,Min Zhu,Zongli Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Survivin ELISA Kit

ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Sandwich ELISA - Human Survivin ELISA Kit (AB183361)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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