Human Synapsin I ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synapsin I in Cell Lysate, Tissue Extracts samples.
Colorimetric
Cell Lysate, Tissue Extracts
Sandwich (quantitative)
Human
187.5 - 12000 pg/mL
1h 30m
= 23.288 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).
Synapsin-1, Brain protein 4.1, Synapsin I, SYN1
Human Synapsin I ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synapsin I in Cell Lysate, Tissue Extracts samples.
Colorimetric
Cell Lysate, Tissue Extracts
Sandwich (quantitative)
Human
187.5 - 12000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 23.288 pg/mL
96%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 8.4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 6.61 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell Lysate | Average % = 96 | Range 91 - 105 % |
Sample type Tissue Extracts | Average % = 105 | Range 91 - 114 % |
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Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.
Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.
Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.
Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.
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Example of human Synapsin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentration of native Synapsin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse brain tissue extract 0.5 µg/mL, rat brain tissue extract 0.5 µg/mL, and human cortex tissue extract 2 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR.
Interpolated concentration of native Synapsin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: SH-SY5Y cell extract 50 µg/mL, and human cortex tissue extract 3 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR.
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