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Human Synapsin I ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synapsin I in Cell Lysate, Tissue Extracts samples.

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Images

Sandwich ELISA - Human Synapsin I ELISA Kit (AB308538), expandable thumbnail
  • Sandwich ELISA - Human Synapsin I ELISA Kit (AB308538), expandable thumbnail
  • Sandwich ELISA - Human Synapsin I ELISA Kit (AB308538), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Sensitivity

= 23.288 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Synapsin I Capture Antibody
1 x 600 µL
10X Human Synapsin I Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human Synapsin I Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Synapsin I ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synapsin I in Cell Lysate, Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 23.288 pg/mL

General recovery

96%

Precision

Intra assay

Sample

Extract

n
8
mean
-
SD
-
C.V.

8.4

Inter assay

Sample

Extract

n
3
mean
-
SD
-
C.V.

6.61

Recovery

Sample specific recovery

Sample type

Cell Lysate

Average %

= 96

Range

91 - 105 %

Sample type

Tissue Extracts

Average %

= 105

Range

91 - 114 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Associated diseases and disorders

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538), expandable thumbnail

    Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538)

    Example of human Synapsin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538), expandable thumbnail

    Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538)

    Interpolated concentration of native Synapsin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse brain tissue extract 0.5 µg/mL, rat brain tissue extract 0.5 µg/mL, and human cortex tissue extract 2 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR.

  • Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538), expandable thumbnail

    Sandwich ELISA - Human Synapsin I ELISA Kit (ab308538)

    Interpolated concentration of native Synapsin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: SH-SY5Y cell extract 50 µg/mL, and human cortex tissue extract 3 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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