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Human Synaptophysin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synaptophysin in Cell Lysate, Tissue Extracts samples.

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Images

Sandwich ELISA - Human Synaptophysin ELISA Kit (AB315042), expandable thumbnail
  • Sandwich ELISA - Human Synaptophysin ELISA Kit (AB315042), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Sensitivity

= 17.511 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Synaptophysin Capture Antibody
1 x 600 µL
10X Human Synaptophysin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human Synaptophysin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Synaptophysin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Synaptophysin in Cell Lysate, Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 17.511 pg/mL

General recovery

110%

Precision

Intra assay

Sample

Extract

n
8
mean
-
SD
-
C.V.

4.7

Inter assay

Sample

Extract

n
3
mean
-
SD
-
C.V.

2.54

Recovery

Sample specific recovery

Sample type

Cell Lysate

Average %

= 110

Range

104 - 114 %

Sample type

Tissue Extracts

Average %

= 102

Range

82 - 114 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Synaptophysin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Synaptophysin protein in Cell Lysate, Tissue extracts. Quantitate Human Synaptophysin with 17.511 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.

Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Associated diseases and disorders

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.

Product promise

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2 product images

  • Sandwich ELISA - Human Synaptophysin ELISA Kit (ab315042), expandable thumbnail

    Sandwich ELISA - Human Synaptophysin ELISA Kit (ab315042)

    Interpolated concentration of native Synaptophysin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human brain tissue extract 1.5 μg/mL and SH-SY5Y cell extract 200 μg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. 

  • Sandwich ELISA - Human Synaptophysin ELISA Kit (ab315042), expandable thumbnail

    Sandwich ELISA - Human Synaptophysin ELISA Kit (ab315042)

    Example of Human Synaptophysin standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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