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Human Syndecan-1 ELISA Kit (CD138) is a Sandwich (quantitative) ELISA kit for the measurement of Human Syndecan-1 (CD138) in Human in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

8 - 256 ng/mL

Assay time

1h 45m

Sensitivity

= 4.94 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).

Alternative names

What's included?

2 x 96 Tests
Components
10X Standard Diluent Buffer
1 x 25 mL
200X Wash Buffer
2 x 10 mL
Biotinylated Antibody Diluent
1 x 13 mL
Biotinylated anti-Syndecan-1
2 x 400 µL
Chromogen TMB Substrate Solution
1 x 24 mL
Control
4 x 1 Vial
HRP Diluent
1 x 23 mL
Plastic Plate Covers
4 x 1 Unit
Stop Reagent
2 x 11 mL
Streptavidin-HRP
4 x 5 µL
Syndecan-1 Microplate (12 x 8 well strips)
2 x 1 Unit
Syndecan-1 Standard (lyophilized)
4 x 1 Vial

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Human Syndecan-1 ELISA Kit (CD138) is a Sandwich (quantitative) ELISA kit for the measurement of Human Syndecan-1 (CD138) in Human in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

8 - 256 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

Sensitivity

= 4.94 ng/mL

General recovery

101%

Precision

Intra assay

Sample

Overall

n

6

mean

-

SD

-

C.V.

6.2

Inter assay

Sample

Overall

n

6

mean

-

SD

-

C.V.

10.2

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.

This kit will recognize both endogenous and recombinant Human Syndecan-1.

Supplementary info

Biological function summary

Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.

Activity summary

Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.

Pathways

Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.

Associated diseases and disorders

Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506), expandable thumbnail

    ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)

    Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).

  • ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506), expandable thumbnail

    ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)

    Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).

  • ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506), expandable thumbnail

    ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)

    Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).

  • Sandwich ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506), expandable thumbnail

    Sandwich ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)

    Representative Standard Curve using ab46506

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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