Human Syndecan-1 ELISA Kit (CD138) is a Sandwich ELISA used to quantify Human Syndecan-1 (CD138) with a sensitivity of 4.94 ng/ml
- Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader
- Wide dynamic range – 8 - 256 ng/ml
- Cited in over 30 citations
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Publications
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- BMC pediatrics 24:682024The association between prolonged capillary refill time and microcirculation changes in children with sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime Fernández-Sarmiento,Shirley Lamprea,Sofia Barrera,Lorena Acevedo,Catalina Duque,Manuela Trujillo,Valeria Aguirre,Carolina JimenezPubMed 38245695
- Microcirculation (New York, N.Y. : 1994) 30:e128292023The association between hypoalbuminemia and microcirculation, endothelium, and glycocalyx disorders in children with sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime Fernández-Sarmiento,Ricardo Hernández-Sarmiento,María Paula Salazar,Sofia Barrera,Valeria Castilla,Catalina DuquePubMed 37639384
- Science advances 9:eadf66002023Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Abdullah,Michael Ghio,Aaron Cotton-Betteridge,Aditya Vinjamuri,Robert Drury,Jacob Packer,Oguz Aras,Jessica Friedman,Mardeen Karim,David Engelhardt,Emma Kosowski,Kelby Duong,Farhana Shaheen,Patrick R McGrew,Charles T Harris,Robert Reily,Mimi Sammarco,Partha K Chandra,Derek Pociask,Jay Kolls,Prasad V Katakam,Alison Smith,Sharven Taghavi,Juan Duchesne,Olan Jackson-WeaverPubMed 37315138
- Nature communications 14:16382023Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Druzak,Elizabeth Iffrig,Blaine R Roberts,Tiantian Zhang,Kirby S Fibben,Yumiko Sakurai,Hans P Verkerke,Christina A Rostad,Ann Chahroudi,Frank Schneider,Andrew Kam Ho Wong,Anne M Roberts,Joshua D Chandler,Susan O Kim,Mario Mosunjac,Marina Mosunjac,Rachel Geller,Igor Albizua,Sean R Stowell,Connie M Arthur,Evan J Anderson,Anna A Ivanova,Jun Ahn,Xueyun Liu,Kristal Maner-Smith,Thomas Bowen,Mirko Paiardini,Steve E Bosinger,John D Roback,Deanna A Kulpa,Guido Silvestri,Wilbur A Lam,Eric A Ortlund,Cheryl L MaierPubMed 37015925
- Life (Basel, Switzerland) 13:2023Syndecan-1: From a Promising Novel Cardiac Biomarker to a Surrogate Early Predictor of Kidney and Liver Injury in Patients with Acute Heart Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRadu-Stefan Miftode,Irina-Iuliana Costache,Daniela Constantinescu,Ovidiu Mitu,Amalia-Stefana Timpau,Monica Hancianu,Daniela-Anicuta Leca,Ionela-Larisa Miftode,Raul-Alexandru Jigoranu,Alexandru-Florinel Oancea,Mihai Stefan Cristian Haba,Diandra Ioana Miftode,Ionela-Lacramioara SerbanPubMed 37109427
- Arthritis research & therapy 25:362023Elevated serum immunoglobulin level predicts high risk of 1-year recurrence in patients with Takayasu arteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanqiu Guo,Juan Du,Taotao Li,Na Gao,Shiyu Yang,Yaxin Zhang,Lili PanPubMed 36882846
- Research and practice in thrombosis and haemostasis 7:1000102022Effects of convalescent plasma infusion on the ADAMTS13-von Willebrand factor axis and endothelial integrity in patients with severe and critical COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuan Zhang,Zhan Ye,Paul McGowan,Christopher Jurief,Andrew Ly,Antonia Bignotti,Noritaka Yada,X Long ZhengPubMed 36531671
- CNS neuroscience & therapeutics 28:1648-16502022Dynamics of intracranial and peripheral plasma Syndecan-1 after ischemic stroke with large vessel occlusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTengkun Yin,Jiheng Hao,Qunlong Jiang,Xin Xu,Bin Xu,Hang Lv,Weidong Liu,Yilei Xiao,Liqun Jiao,Jiyue Wang,Liyong ZhangPubMed 35790077
- Kidney international reports 7:797-8092022Minimal Change Disease Is Associated With Endothelial Glycocalyx Degradation and Endothelial Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColin Bauer,Federica Piani,Mindy Banks,Flor A Ordoñez,Carmen de Lucas-Collantes,Kaori Oshima,Eric P Schmidt,Igor Zakharevich,Alfons Segarra,Cristina Martinez,Carlos Roncal-Jimenez,Simon C Satchell,Petter Bjornstad,Marshall Scott Lucia,Judith Blaine,Joshua M Thurman,Richard J Johnson,Gabriel Cara-FuentesPubMed 35497798
- PLoS neglected tropical diseases 16:e00100512022Effect of oseltamivir phosphate versus placebo on platelet recovery and plasma leakage in adults with dengue and thrombocytopenia; a phase 2, multicenter, double-blind, randomized trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRahajeng N Tunjungputri,Silvita Fitri Riswari,Setyo G Pramudo,Lydia Kuntjoro,Bachti Alisjahbana,Harry Galuh Nugraha,Andre van der Ven,Muhammad Hussein Gasem,Quirijn de MastPubMed 34995275
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8 - 256 ng/mL
- Assay time
- 1h 45m
- Sensitivity
- = 4.94 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the SDC1 gene is a cell surface proteoglycan with heparan sulfate and chondroitin sulfate, linking the cytoskeleton to the interstitial matrix. It regulates exosome biogenesis alongside SDCBP and PDCD6IP. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD138, SDC, SDC1, Syndecan-1, SYND1
What's included?
Recommended products
Human Syndecan-1 ELISA Kit (CD138) is a Sandwich ELISA used to quantify Human Syndecan-1 (CD138) with a sensitivity of 4.94 ng/ml
- Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader
- Wide dynamic range – 8 - 256 ng/ml
- Cited in over 30 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8 - 256 ng/mL
- Assay time
- 1h 45m
- Assay Platform
- Microplate
- Sensitivity
- = 4.94 ng/mL
- General recovery
- 101%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 6 | C.V. 6.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 6 | C.V. 10.2 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
Human Syndecan-1 ELISA Kit (CD138) ab46506 is a sandwich ELISA to measure Human Syndecan (CD138) in serum, plasma, buffered solutions and supernatants. with a sensitivity of 4.94 ng/mL.
How the assay works
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
Assay specificity
This kit will recognize both endogenous and recombinant Human Syndecan-1.
Human Syndecan-1 ELISA Kit (CD138) ab46506 protocol summary
1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
4. Aspirate and wash each well. Add the TMB Solution to each well until color develops and then add the Stop Solution. Immediately begin recording the color development
How other researchers are using Human Syndecan-1 ELISA Kit (CD138) ab46506
Human Syndecan-1 ELISA Kit (CD138) ab46506 has been used to measure endothelial glycocalyx degradation in children with sepsis (1) and in traumatic hemorrage (2).
References
(1)J Fernandez-Sarmiento et al. 2024, PMID:38245695
(2) S. Abdullah et al. 2023, PMID: 37315138
Related and recommended products
Human Syndecan-1 ELISA Kit (CD138) ab46506 is commonly used to study inflammation, endothelial dysfunction and other cellular processes.
Other ELISA kits commonly use alongside Syndecan-1 include:
- Human VEGF ELISA Kit Human VEGF ELISA Kit ab222510
- Human IL-6Elisa Kit Human IL-6 ELISA Kit ab178013
- Human MMP9 ELISA Kit Human MMP9 ELISA Kit ab246539
- Human ICAM1 ELISA Kit (CD54) Human ICAM1 ELISA Kit (CD54) ab174445
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
- Biological function summary
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
- Pathways
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
- Associated diseases and disorders
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.
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4 product images
ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).
ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).
ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Human Syndecan-1 ELISA Kit (CD138) (ab46506)
Representative Standard Curve using ab46506
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