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Human Syndecan-1 ELISA Kit (CD138) is a Sandwich (quantitative) ELISA kit for the measurement of Human Syndecan-1 (CD138) in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
8 - 256 ng/mL
1h 45m
= 4.94 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).
Syndecan-1, SYND1, SDC, SDC1
Human Syndecan-1 ELISA Kit (CD138) is a Sandwich (quantitative) ELISA kit for the measurement of Human Syndecan-1 (CD138) in Human in Plasma, Cell culture supernatant, Serum samples.
Syndecan-1, SYND1, SDC, SDC1
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
8 - 256 ng/mL
1h 45m
Microplate
= 4.94 ng/mL
101%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 6 | mean - | SD - | C.V. 6.2 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 6 | mean - | SD - | C.V. 10.2 |
Blue Ice
+4°C
+4°C
+4°C
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).
Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).
Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab46506
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