AB213830

Human Syndecan-4 ELISA Kit (SDC4)

Name: Human Syndecan-4 ELISA Kit (SDC4) (ab213830) | Abcam
Brand: Abcam Limited
SKU: AB213830
Price: 755 USD
Availability: InStock

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(1 Publication)

Human Syndecan-4 ELISA Kit (SDC4) is a Sandwich ELISA for the measurement of Human Syndecan-4 (SDC4) in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Syndecan-4, SYND4, Amphiglycan, Ryudocan core protein, SDC4

1 Images

Sandwich ELISA - Human Syndecan-4 ELISA Kit (SDC4) (AB213830)

sELISA

Supplier Data

Sandwich ELISA - Human Syndecan-4 ELISA Kit (SDC4) (AB213830)

Human Syndecan-4 ELISA Kit (SDC4) (ab213830) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Human Syndecan-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (SDC4) (ab213830) is designed for the quantitative measurement of Human Syndecan-4 in cell culture supernatants, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-4/SDC4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E19-E145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-4/SDC4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Syndecan-4/SDC4 amount of sample captured in plate.

Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecan's and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What's more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "680 pg/mL", "standardDeviation": "680", "coefficientOfVariability": "= 8.3" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1958 pg/mL", "standardDeviation": "1958", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "673 pg/mL", "standardDeviation": "52.49", "coefficientOfVariability": "= 8.3" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2108 pg/mL", "standardDeviation": "128.58", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "94 pg/mL", "standardDeviation": "3.85", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "102 pg/mL", "standardDeviation": "102", "coefficientOfVariability": "= 5.4" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syndecan 4 also known as SDC4 is a transmembrane heparan sulfate proteoglycan with a molecular mass of around 30 kDa. It functions mechanically by interacting with the extracellular matrix via its heparan sulfate chains which are critical for binding growth factors and other matrix molecules. Syndecan 4 is ubiquitously expressed in many cell types including fibroblasts and epithelial cells playing a role in cell adhesion and migration.

Biological function summary

Syndecan 4 contributes to cell signaling and cytoskeletal organization. This proteoglycan acts as an essential co-receptor for various growth factors facilitating their interaction with their receptors on the cell surface. By doing so it supports cell proliferation and repair processes and it's also known to interact with integrins to modulate cell adhesion. Syndecan 4 does not function alone; it forms part of complexes with other cell surface receptors and interacts with key signaling molecules inside cells.

Pathways

Syndecan 4 is integral in the regulation of several signal transduction pathways such as the protein kinase C (PKC) and Rho family GTPase pathways. These pathways are essential for modulating cellular responses to mechanical stress and changes in the extracellular environment. Syndecan 4 works closely with proteins such as fibronectin and integrins within these pathways to distribute mechanical signals appropriately across the cell membrane linking the extracellular matrix to intracellular processes.

Syndecan 4 has strong associations with conditions like cancer and arthritis. In cancer altered Syndecan 4 expression can impact tumor cell proliferation and metastasis often in concert with matrix metalloproteinases which remodel the extracellular matrix environment to facilitate invasion. In arthritis Syndecan 4 plays a role in inflammatory processes where its interactions with cytokines and other cell-surface proteins can influence cartilage degradation and joint inflammation.

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Product protocols

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Target data

Cell surface proteoglycan which regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed : 22660413).

See full target information SDC4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:5793912 PubMed35937409

2022

LINC00707 Promotes Cell Proliferation in Cervical Cancer via the miR-374c-5p/SDC4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fang,Chunfeng Guo,Weinan Zheng,Qingyang Li

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Syndecan-4 ELISA Kit (SDC4)

Sandwich ELISA - Human Syndecan-4 ELISA Kit (SDC4) (AB213830)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise