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AB316901

Human Syntaxin 1B ELISA Kit

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Human Syntaxin 1B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Syntaxin 1B with a sensitivity of 16.949 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

STX1B1, STX1B2, STX1B, Syntaxin-1B, Syntaxin-1B1, Syntaxin-1B2

3 Images
Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)
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Supplier Data

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)

Example of human Syntaxin 1B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)
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Supplier Data

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)

Interpolated concentrations of human Syntaxin 1B in SH-SY5Y cell extract and human cortex, brain stem, and hippocampus tissue extracts.

Interpolated concentration of native Syntaxin 1B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 500 µg/mL SH-SY5Y cell extract, 0.5 µg/mL human cortex tissue extract, 5 µg/mL human brain stem tissue extract, and 1 µg/mL human hippocampus tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Syntaxin 1B ELISA Kit (AB316901)

Interpolated concentrations of Syntaxin 1B in mouse brain and rat brain tissue extracts

Interpolated concentration of native Syntaxin 1B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 2 µg/mL mouse brain tissue extract and 1 µg/mL rat brain tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16.949 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Syntaxin 1B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Syntaxin 1B protein in Cell Lysate, Tissue extracts. Quantitate Human Syntaxin 1B with 16.949 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.2" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "79 - 84 %", "average": "= 81" }, { "sample": "Tissue Extracts", "range": "86 - 101 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntaxin 1B often abbreviated as STX1B is an essential SNARE protein with a mass of approximately 33 kDa. This protein is mainly expressed in neurons and tissues with neural characteristics indicating its prominent role in the nervous system. Mechanically Syntaxin 1B facilitates the docking and fusion of synaptic vesicles with the presynaptic membrane. Through this mechanism it aids in the release of neurotransmitters which is fundamental for synaptic transmission and communication between neurons.
Biological function summary

This protein functions as a core component of the SNARE complex comprising Syntaxin 1B SNAP-25 and VAMP2. The formation of this complex brings the synaptic vesicle and presynaptic membrane into close proximity creating the condition for vesicle fusion. Syntaxin 1B plays a significant role in synaptic efficiency and plasticity impacting processes such as learning and memory.

Pathways

Syntaxin 1B is integral to the neurotransmitter release pathway and exocytosis pathways. It closely interacts with proteins such as SNAP-25 and VAMP2 both of which are critical for the proper execution of synaptic vesicle release. Within these pathways Syntaxin 1B assists in the regulated release of chemical messengers ultimately influencing neuronal communication and overall neural network activity.

Mutations or dysfunctions in Syntaxin 1B have links to neurological conditions such as epilepsy and intellectual disability. Research indicates that abnormalities in Syntaxin 1B might disrupt synaptic transmission potentially through altered interactions with proteins like SNAP-25. These disruptions can lead to erratic neuronal firing patterns or inadequate synaptic connectivity contributing to the pathophysiology observed in these disorders.
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Product protocols

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Target data

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm (By similarity).
See full target information STX1B
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB314153

Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector) (AB314153)

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