AB315038

Human Syntenin ELISA Kit

SimpleStep
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Human Syntenin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Syntenin with a sensitivity of 19.95 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MDA9, SYCL, SDCBP, Syntenin-1, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, Syndecan-binding protein 1, MDA-9, TACIP18

3 Images

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

sELISA

Supplier Data

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Example of human Syntenin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Interpolated concentration of native Syntenin was measured in duplicate at different sample concentrations. Undiluted sample is 30 µg/mL SK-MEL-28 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Representative image of Human Syntenin ELISA Kit (ab315038)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Syntenin ELISA Kit Capture Antibody

- 10X Human Syntenin Detector Antibody

- 10X Human Syntenin ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Syntenin ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 19.95 pg/mL

Range

187.5 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Syntenin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Syntenin protein in Cell Lysate. Quantitate Human Syntenin with 19.95 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "87 - 105 %", "average": "= 94" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315038-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315038-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315038-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315038-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315038-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315038-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Syntenin Lyophilized Recombinant Protein", "number":"AB315038-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB315038-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Syntenin Capture Antibody", "number":"AB315038-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Syntenin Detector Antibody", "number":"AB315038-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315038-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315038-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315038-CMP12", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syntenin also known as syndecan binding protein (SDCBP) is a multifunctional adaptor protein with a molecular weight of approximately 32 kDa. It typically finds expression in various tissues with high levels noted in the brain placenta and kidney. Mechanically syntenin facilitates cellular signal transduction by interacting with the cytoplasmic domains of syndecans therefore playing a vital role in modulating those signals. Notably syntenin associates with the actin cytoskeleton impacting cell shape and motility.

Biological function summary

Syntenin regulates important cellular processes such as cell adhesion migration and intracellular trafficking. It often associates with protein complexes like those formed with PDZ domain-containing proteins enhancing its ability to link and organize diverse cellular pathways. By influencing these cellular processes syntenin has an impact on tissue development and repair mechanisms. This protein's interaction with the cytoskeleton and membrane-bound molecules allows it to act as an essential mediator in cellular communication.

Pathways

Syntenin is integral to the Wnt and TGF-beta signaling pathways which regulate cell growth and differentiation. Syntenin interacts with proteins such as β-catenin and Smad3 within these pathways linking extracellular signaling cues to specific intracellular responses. These interactions highlight syntenin's role in modulating gene expression and maintaining cellular homeostasis especially in processes like embryogenesis and tissue regeneration.

Syntenin holds significance in cancer progression and neurodegenerative diseases. Its overexpression or altered function correlates with increased metastasis in various cancers associated with proteins like syndecan-1 and matrix metalloproteinases. In neurodegeneration dysregulated syntenin expression influences signaling pathways potentially contributing to disorders like Alzheimer's disease. Understanding syntenin's role in these conditions might lead to more targeted therapeutic strategies and improved disease management.

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Product protocols

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Target data

Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed : 26291527). Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed : 25893292). In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed : 22660413). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed : 26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed : 11498591). May also play a role in vesicular trafficking (PubMed : 11179419). Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed : 10230395).

See full target information SDCBP

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Syntenin ELISA Kit

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Sandwich ELISA - Human Syntenin ELISA Kit (AB315038)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Proteins & Peptides

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Product promise