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Human TACE ELISA Kit (ADAM17) is a Sandwich (quantitative) ELISA kit for the measurement of Human TACE (ADAM17) in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17, TACE, CSVP
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
78.15 - 5000 pg/mL
- Sensitivity
< 70 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human TACE ELISA Kit (ADAM17) is a Sandwich (quantitative) ELISA kit for the measurement of Human TACE (ADAM17) in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17, TACE, CSVP
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
78.15 - 5000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 70 pg/mL
- General recovery
100%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98.65 | Range 89 - 124 % |
Sample type Serum | Average % = 112.3 | Range 85 - 132 % |
Sample type Plasma | Average % = 101.1 | Range 72 - 128 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
The standard is affinity purified.
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