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AB288592

Human TACI ELISA Kit

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Human TACI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TACI with a sensitivity of 2.28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD267, TACI, TNFRSF13B, Tumor necrosis factor receptor superfamily member 13B, Transmembrane activator and CAML interactor

5 Images
Sandwich ELISA - Human TACI ELISA Kit (AB288592)
  • sELISA

Supplier Data

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

Standard curve comparison between the original Human TACI SimpleStep ELISA (ab193766) and current Human TACI SimpleStep ELISA (ab288592).

Sandwich ELISA - Human TACI ELISA Kit (AB288592)
  • sELISA

Supplier Data

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean TACI concentration was determined to be 115.6 pg/mL with a range of 80.1 - 158.8 pg/mL.

Sandwich ELISA - Human TACI ELISA Kit (AB288592)
  • sELISA

Supplier Data

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

Interpolated concentration of native TACI was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (heparin), plasma (EDTA), plasma (citrate), cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human TACI ELISA Kit (AB288592)
  • sELISA

Supplier Data

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

Representative image of Human TACI ELISA Kit (ab288592)

Components shown from left to right :

- Sample diluent NS

- 10X Human TACI Capture Antibody

- 10X Human TACI Detector Antibody

- Human TACI Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Sandwich ELISA - Human TACI ELISA Kit (AB288592)
  • sELISA

Supplier Data

Sandwich ELISA - Human TACI ELISA Kit (AB288592)

Example of human TACI standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.28 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TACI ELISA Kit (ab288592) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TACI protein in Cerebral spinal fluid, urine, serum, EDTA plasma, Hep plasma, Cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TACI with 2.28 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 107 %", "average": "= 103" }, { "sample": "Serum", "range": "111 - 116 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "111 - 113 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "114 - 116 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "103 - 111 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288592-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB288592-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288592-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288592-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288592-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB288592-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288592-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288592-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288592-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TACI Capture Antibody", "number":"AB288592-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TACI Lyophilized Recombinant Protein", "number":"AB288592-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TACI Detector Antibody", "number":"AB288592-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transmembrane Activator and CAML Interactor known as TACI also goes by the names TNFRSF13B and CD267. TACI is a receptor with a molecular weight of approximately 33 kDa. It is expressed on B cells a type of lymphocyte and to some extent on activated T cells. This receptor plays a part in the immune system helping manage the production and function of certain antibodies.
Biological function summary

TACI involves the regulation of B cell function and survival. It binds to two ligands APRIL and BAFF and TACI is not part of any larger complex. Upon ligand binding TACI can promote antibody class-switching which adjusts the type of antibody that B cells produce. This action supports the immune response by allowing the body to adapt to different pathogens effectively.

Pathways

Immune responses involve TACI in the BAFF-APRIL signaling pathway. The interplay between TACI and its ligands influences the NF-kB signaling cascade leading to B cell proliferation and survival. TACI interacts with other proteins such as BCMA and BAFF-R which are important in regulating B cell homeostasis and function ensuring balanced and effective immune responses.

Aberrant TACI function contributes to conditions like Common Variable Immunodeficiency (CVID) and Systemic Lupus Erythematosus (SLE). In CVID mutations in the TACI gene may lead to impaired antibody production resulting in frequent infections. In the context of SLE TACI's interactions with BAFF and APRIL can contribute to the overactivation of B cells causing autoimmune characteristics of the disease. TACI's role in these conditions makes it a focus for studying therapeutic interventions.
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Product protocols

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Target data

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.
See full target information TNFRSF13B
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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