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Human TAFA1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TAFA1 in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human TAFA1 ELISA Kit (AB314359), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.12 - 25 ng/mL
Sensitivity
= 0.12 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Regulatory factor which is ligand for CMKLR2 and is involved in the modulation of neural stem-cell proliferation and differentiation.

Alternative names

FAM19A1, TAFA1, Chemokine-like protein TAFA-1

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
400X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
Biotinylated Human TAFA1 Detection Antibody
2 x 1 Vial
Human TAFA1 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human TAFA1 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Human TAFA1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TAFA1 in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.12 - 25 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.12 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 80.4
Range
71 - 90 %
Sample type
Plasma
Average %
= 71.79
Range
69 - 76 %
Sample type
Cell culture media
Average %
= 89.55
Range
83 - 94 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human TAFA1 (FAM19A1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TAFA1 in serum, plasma (Collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human TAFA1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO2, Arginase 1, B7-H4, Bcl-10, CD42b, CD73, CES1, CES2, cIAP-1, Cyclophilin A, Cystatin S, DNMT3A, Epimorphin, GDF-9, Glypican 3, GPR115, HE4, HO-1, HS3ST4, IGSF3, IL-17 RD, Integrin alpha 1, KIR2DL3, LAMP2, LEDGF, MOG, Nestin, Neudesin, Neuroligin 2, NKp80, Osteoadherin, PDGF R alpha, PRDX4, Syntaxin 4,TAFA2, TAFA5, Tenascin R, TGM4, TMEFF1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human TAFA1 ELISA Kit (ab314359), expandable thumbnail

    Sandwich ELISA - Human TAFA1 ELISA Kit (ab314359)

    Example of standard curve using ab314359. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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