Human Talin 1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human Talin 1 with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.5 - 32 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.22 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.
Alternative names
KIAA1027, TLN, TLN1, Talin-1
What's included?
Recommended products
Human Talin 1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human Talin 1 with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.5 - 32 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.22 ng/mL
- General recovery
- 80%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 4.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 6.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 80 | Range 75 - 86 % |
Sample type Citrate plasma | Average % = 86 | Range 78 - 95 % |
Sample type EDTA Plasma | Average % = 82 | Range 80 - 84 % |
Sample type Cell culture media | Average % = 87 | Range 83 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Talin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Talin 1 protein in Serum, Cit Plasma, EDTA Plasma, and Cell culture media. Quantitate Human Talin 1 with 0.22 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.
- Biological function summary
Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.
- Pathways
Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.
- Associated diseases and disorders
Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.
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5 product images
Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (ab315317)
Example of human Talin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (ab315317)
Serum samples from mouse, rat, monkey, cow, and fetal cow species were measured in duplicate. Protein concentrations were interpolated from the human standard curve. Interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (ab315317)
Recombinant Talin 1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 12.5% serum, plasma (citrate), plasma (EDTA), and cell culture media (RPMI1640 containing no fetal bovine serum). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (ab315317)
Interpolated concentration of native Talin 1 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% serum, plasma (citrate) and plasma (EDTA). The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (ab315317)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Talin 1 concentration of detected samples was determined to be 7.76 ng/mL with a range of undetected – 14.11 ng/mL.
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