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AB315317

Human Talin 1 ELISA Kit- extracellular

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Human Talin 1 ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human Talin 1 with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

KIAA1027, TLN, TLN1, Talin-1

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Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Example of human Talin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Interpolated concentration of native Talin 1 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% serum, plasma (citrate) and plasma (EDTA). The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Recombinant Talin 1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 12.5% serum, plasma (citrate), plasma (EDTA), and cell culture media (RPMI1640 containing no fetal bovine serum).  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Talin 1 concentration of detected samples was determined to be 7.76 ng/mL with a range of undetected – 14.11 ng/mL.

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Serum samples from mouse, rat, monkey, cow, and fetal cow species were measured in duplicate. Protein concentrations were interpolated from the human standard curve. Interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- extracellular (AB315317)

Representative image of Human Talin 1 ELISA Kit- extracellular (ab315317)

Components shown from left to right :

- Sample diluent NS (2 bottles)

- 10X Human Talin 1 Capture Antibody

- 10X Human Talin 1 Detector Antibody

- Human Talin 1 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPI2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.22 ng/mL

Range

0.5 - 32 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Talin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Talin 1 protein in Serum, Cit Plasma, EDTA Plasma, and Cell culture media. Quantitate Human Talin 1 with 0.22 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.2" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 86 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "78 - 95 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "80 - 84 %", "average": "= 82" }, { "sample": "Cell culture media", "range": "83 - 91 %", "average": "= 87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315317-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315317-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315317-CMP09", "productcode":"" }, { "size":"1 x 100 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315317-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315317-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Talin 1 Lyophilized Recombinant Protein", "number":"AB315317-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315317-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB315317-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Talin 1 Capture Antibody", "number":"AB315317-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Talin 1 Detector Antibody", "number":"AB315317-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315317-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315317-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.
Biological function summary

Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.

Pathways

Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.

Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.
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Product protocols

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Target data

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.
See full target information TLN1
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websiteProtocolBooklet
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