JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315319

Human Talin 1 ELISA Kit- intracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human Talin 1 ELISA Kit- intracellular is a single-wash 90-min Simplestep used to quantify Human Talin 1 with a sensitivity of 0.044 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

KIAA1027, TLN, TLN1, Talin-1

2 Images
Sandwich ELISA - Human Talin 1 ELISA Kit- intracellular (AB315319)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- intracellular (AB315319)

Interpolated concentration of native Talin 1 was measured in duplicate at different sample concentrations. Undiluted samples are 100 µg/mL HeLa cell extract, 250 µg/mL NIH/3T3 cell extract, and 75 µg/mL C6 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Talin 1 ELISA Kit- intracellular (AB315319)
  • sELISA

Supplier Data

Sandwich ELISA - Human Talin 1 ELISA Kit- intracellular (AB315319)

Example of Talin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.044 ng/mL

Range

0.156 - 10 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB71333

Anti-Talin 1 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody (AB71333)

  • 4
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Talin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Talin 1 protein in Cell Lysate. Quantitate Human Talin 1 with 0.044 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "85 - 96 %", "average": "= 92" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315319-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315319-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315319-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315319-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315319-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Talin 1 Lyophilized Recombinant Protein", "number":"AB315319-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB315319-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315319-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Talin 1 Capture Antibody", "number":"AB315319-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Talin 1 Detector Antibody", "number":"AB315319-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315319-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315319-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315319-CMP12", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.
Biological function summary

Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.

Pathways

Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.

Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.
style
left-column

Product protocols

style
left-column

Target data

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.
See full target information TLN1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com