AB183366

Human TARC ELISA Kit (CCL17)

SimpleStep
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(2 Publications)

Human TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Human TARC (CCL17) with a sensitivity of 78.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine

3 Images

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Titration of PBMC Cell Culture Supernatants diluted within the working range of the assay.

Background subtracted data from duplicate measurements are plotted. Bar graph denotes quantification of TARC interpolated from standard curve and multiplied by dilution factor.

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Example of TARC standard curve.

The TARC standard curve was prepared using Sample Diluent. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Example of TARC standard curve.

The TARC standard curve was prepared using Sample Diluent. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 78.5 pg/mL

Range

176 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TARC ELISA Kit (CCL17) (ab183366) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TARC protein in human cell culture supernatants, serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human TARC with 78.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

TARC is a member of the CC chemokine group that is constitutively expressed in the thymus and is transiently produced by a variety of cell types: dendritic cells (DC), endothelial cells, keratinocytes (KC), bronchial epithelial cells and fibroblasts. TARC is a ligand for chemokine receptor CCR4, expressed on Th2 lymphocytes, basophils and natural killer cells. CCL17 binds to CCR4 to induce chemotaxis in T cells.

TARC production appears to be upregulated by TNF-α and IFN-γ synergistically through NFkB and p38 signaling pathways in Human kertinocytes. Cytokines IL-4 and TGF-β1 act to downregulate TNF-α and IFN-γ induced TARC production in Human keratinocytes. TARC (CCL17) and its receptor CCR4 are believed to play important roles in the pathogenesis of the skin diseases Atopic Dermatitis (AD), Bullous pemphigoid (BP) and Mycosis fungoides (MF).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "91 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "97 - 98 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "104 - 111 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "116 - 130 %", "average": "= 123" }, { "sample": "Serum", "range": "98 - 126 %", "average": "= 112" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.

Biological function summary

CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.

Pathways

CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.

CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.

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Product protocols

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Target data

Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed : 8702936, PubMed : 9169480). Acts by binding to CCR4 at T-cell surface (PubMed : 10540332, PubMed : 9169480). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed : 27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (By similarity). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (By similarity).

See full target information CCL17

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Publications (2)

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Experimental and therapeutic medicine 26:359 PubMed37324510

2023

CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Wen Gao,Han-Lin Hu,Ming-Hua Xie,Cai-Xia Tang,Jun Ou,Zheng-Hao Lu

Cancer management and research 11:7439-7453 PubMed31496803

2019

Down-regulation of CCL17 in cancer-associated fibroblasts inhibits cell migration and invasion of breast cancer through ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Li,Chunli Yang,Jingshi Yang,Liqun Zou

View all publications

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Product promise

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Human TARC ELISA Kit (CCL17)

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Sandwich ELISA - Human TARC ELISA Kit (CCL17) (AB183366)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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