AB282307

Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5)

Recombinant
SimpleStep
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Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) is a single-wash 90-min Simplestep used to quantify Human Tartrate Resistant Acid Phosphatase (ACP5) with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tartrate-resistant acid phosphatase type 5, TR-AP, Tartrate-resistant acid ATPase, Type 5 acid phosphatase, TrATPase, ACP5

3 Images

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

sELISA

Supplier Data

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tartrate Resistant Acid Phosphatase concentration was determined to be 17,030 pg/mL with a range of 3,727 – 66,076 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Interpolated concentrations of native Tartrate Resistant Acid Phosphatase in human serum, plasma (citrate, EDTA and heparin) and MDA-MB-435 cell culture supernatant samples.

The concentrations of Tartrate Resistant Acid Phosphatase were measured in duplicates, interpolated from the Tartrate Resistant Acid Phosphatase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and MDA-MB-435 supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tartrate Resistant Acid Phosphatase concentration was determined to be 13,156 pg/mL in neat serum, 20,691 pg/mL in neat plasma (citrate), 12888 pg/mL in neat plasma (EDTA), 13,434 pg/mL in neat plasma (heparin) and 233,970 pg/mL in neat MDA-MB-435 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Example of human Tartrate Resistant Acid Phosphatase standard curve in Sample Diluent NS.

The Tartrate Resistant Acid Phosphatase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tartrate Resistant Acid Phosphatase ELISA kit (ab282307) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tartrate Resistant Acid Phosphatase protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tartrate Resistant Acid Phosphatase with 8 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tartrate Resistant Acid Phosphatase, also known as Type 5 Acid Phosphatase (ACP5) is a lysosomal phosphatase involved in osteopontin/bone sialoprotein dephosphorylation. The expression of Type 5 Acid Phosphatase seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 95 %", "average": "= 89" }, { "sample": "Serum", "range": "89 - 113 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "82 - 96 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "84 - 94 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tartrate Resistant Acid Phosphatase (TRAP) also known as ACP-5 and type 5 acid phosphatase is an enzyme with mass approximately 35 kDa. It is expressed in osteoclasts macrophages and dendritic cells playing a role in bone resorption and macrophage activation. TRAP exists in both a monomeric and proteolytically activated dimeric form. Its unique tartrate resistance distinguishes it from other acid phosphatases providing specificity for enzyme assays and studies.

Biological function summary

TRAP contributes significantly to bone remodeling through its action in osteoclasts. It helps in degrading mineralized matrix components and may also serve a role in dendritic cell activation signaling and macrophage-related processes. TRAP forms part of a complex mechanism that includes catabolic enzymes which work together to ensure normal skeletal function and immune response.

Pathways

TRAP plays an integral role in the bone resorption pathway along with proteins such as cathepsin K and matrix metalloproteinases. It is involved in osteoclast differentiation necessary for bone turnover and homeostasis. Additionally it participates in the immune response through its activity in macrophages affecting inflammation pathways and innate immune functions.

Abnormal TRAP activity associates with osteopetrosis and osteoporosis. These diseases affect bone density and strength with TRAP often showing elevated levels in conditions of high bone turnover. TRAP activity also connects to inflammatory disorders due to its expression in macrophages linking it to proteins like RANKL and osteoprotegerin which influence bone metabolism and immune signaling.

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Product protocols

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Target data

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

See full target information Tartrate-resistant acid phosphatase type 5

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5)

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Sandwich ELISA - Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) (AB282307)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise