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Human Tau (4R isoforms) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (4R isoforms) in Cerebral Spinal Fluid, Cell culture media, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.

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Images

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (AB309282), expandable thumbnail
  • Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (AB309282), expandable thumbnail
  • Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (AB309282), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Sensitivity

= 5.264 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Tau (4R isoforms) Capture Antibody
1 x 600 µL
10X Human Tau (4R isoforms) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human Tau (4R isoforms) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Tau (4R isoforms) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (4R isoforms) in Cerebral Spinal Fluid, Cell culture media, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.264 pg/mL

General recovery

85%

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

8.2

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

7.1

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 85

Range

85 - 86 %

Sample type

Heparin Plasma

Average %

= 98

Range

94 - 100 %

Sample type

Citrate plasma

Average %

= 95

Range

93 - 97 %

Sample type

EDTA Plasma

Average %

= 86

Range

85 - 87 %

Sample type

Cell culture media

Average %

= 105

Range

103 - 108 %

Sample type

Cerebral Spinal Fluid

Average %

= 105

Range

103 - 106 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Tau (4R isoforms) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (4R isoforms) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Tau (4R isoforms) with 5.264 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips


A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282), expandable thumbnail

    Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282)

    50% serum samples of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). Sample from one donor was positive with Tau (4R Isoforms) at a concentration of 347 pg/mL, and the remaining nine samples measured below the detectable range of this assay. 

  • Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282), expandable thumbnail

    Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282)

    Example of human Tau (4R Isoforms) standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282), expandable thumbnail

    Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit (ab309282)

    Recombinant Tau (4R Isoforms) was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows: serum 100%, plasma (citrate) 25%, plasma (EDTA) 50%, plasma (heparin) 50%, 10F RPMI 1640 cell culture media 100%, and CSF 100%. The interpolated dilution factor corrected values to undiluted samples are plotted (mean +/- SD, n=2). 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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