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AB326394

Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular

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Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Tau (4R isoforms) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.83 - 4000 pg/ml with a sensitivity of 0.72pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

5 Images
Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)
  • sELISA

Lab

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)

Interpolated concentrations of Human Tau (4R isoforms) in brain tissue (cortex) extract brain tissue (stem) extract and SH-SY5Y cell extract.

Interpolated concentration of native Tau (4R isoforms) was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Human brain tissue (cortex) extract 2 µg/mL Human brain tissue (stem) extract 10 µg/mL and SH-SY5Y cell extract 15 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)

Interpolated concentrations of Tau (4R isoforms) in mouse and rat brain tissue extracts.

The concentration of Tau (4R isoforms) was measured in 1.0 – 0.125 mg/mL of mouse brain tissue extracts and in 1.25 – 0.31 mg/mL of rat brain tissue extracts in duplicates. The interpolated dilution factor corrected values are plotted in pg of Tau per µg of extract (mean +/- SD). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)
  • sELISA

Lab

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)

Standard curve comparison between the Chemiluminescent Human Tau (4R Isoforms) SimpleStep ELISA and colorimetric Human Tau (4R Isoforms) SimpleStep ELISA (ab303750) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)
  • sELISA

Lab

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (AB326394)

Dynamic range comparison between the Chemiluminescent Human Tau (4R Isoforms) SimpleStep ELISA, colorimetric Human Tau (4R Isoforms) SimpleStep ELISA (ab303750), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.72 pg/mL

Range

1.83 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (ab326394) is a rapid single-wash sandwich 55-min ELISA to measure Human Tau (4R isoforms) in Cell culture extracts, Tissue Extracts with a sensitivity of 0.72 pg/mL.

How the assay works

Human Tau (4R isoforms) ELISA Kit, Chemiluminescent - Intracellular (ab326394) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Tau (4R isoforms) (ab326394) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-4R Tau antibody [EPR24491-216] - BSA and Aside free (Capture) ab309216
- Anti-4R Tau antibody [EPR23025-24] - BSA and Aside free (Detector) ab309217
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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.1" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "109 - 123 %", "average": "= 117" }, { "sample": "Tissue Extracts", "range": "106 - 119 %", "average": "= 111" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT

Additional targets

MAPT,MAPT
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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