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AB309282

Human Tau (4R isoforms) ELISA Kit - Extracellular

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(1 Publication)

Human Tau (4R isoforms) ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Tau (4R isoforms) with a sensitivity of 5.264 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images
Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)

Interpolated concentrations of spiked human Tau (4R Isoforms) in serum, plasma (citrate, EDTA, heparin), cell culture media, and CSF

Recombinant Tau (4R Isoforms) was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : serum 100%, plasma (citrate) 25%, plasma (EDTA) 50%, plasma (heparin) 50%, 10F RPMI 1640 cell culture media 100%, and CSF 100%. The interpolated dilution factor corrected values to undiluted samples are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)

Interpolated concentrations of Tau (4R Isoforms) in normal human serum donors

50% serum samples of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). Sample from one donor was positive with Tau (4R Isoforms) at a concentration of 347 pg/mL, and the remaining nine samples measured below the detectable range of this assay.

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (4R isoforms) ELISA Kit - Extracellular (AB309282)

Example of human Tau (4R Isoforms) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.264 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Tau (4R isoforms) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (4R isoforms) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Tau (4R isoforms) with 5.264 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 86 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "94 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "103 - 108 %", "average": "= 105" }, { "sample": "Cerebral Spinal Fluid", "range": "103 - 106 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT

Additional targets

MAPT,MAPT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 14: PubMed39682654

2024

Serum Tau Species in Progressive Supranuclear Palsy: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Costanza Maria Cristiani,Luana Scaramuzzino,Elvira Immacolata Parrotta,Giovanni Cuda,Aldo Quattrone,Andrea Quattrone
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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