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Human Tau ELISA Kit ab273617 is a single-wash 90-min SimpleStep ELISA used to quantify Human Tau with a sensitivity of 16 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human Tau sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation- Validated in a range of biological samples including CSF

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Images

Sandwich ELISA - Human Tau ELISA Kit (AB273617), expandable thumbnail
  • Sandwich ELISA - Human Tau ELISA Kit (AB273617), expandable thumbnail
  • Sandwich ELISA - Human Tau ELISA Kit (AB273617), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma, Cerebral Spinal Fluid

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

40.63 - 2600 pg/mL

Assay time

1h 30m

Sensitivity

= 16 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Tau Capture Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 5BI
1 x 6 mL
Human Tau Detector Antibody
1 x 600 µL
Human Tau Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Tau ELISA Kit ab273617 is a single-wash 90-min SimpleStep ELISA used to quantify Human Tau with a sensitivity of 16 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human Tau sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation- Validated in a range of biological samples including CSF

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma, Cerebral Spinal Fluid

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

40.63 - 2600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 16 pg/mL

Precision

Intra assay

Sample

Extract

n
8
mean
-
SD
-
C.V.

5.5

Inter assay

Sample

Extract

n
3
mean
-
SD
-
C.V.

2.7

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 79

Range

76 - 84 %

Sample type

EDTA Plasma

Average %

= 78

Range

75 - 83 %

Sample type

Cell culture extracts

Average %

= 97

Range

98 - 101 %

Sample type

Cell culture media

Average %

= 86

Range

84 - 91 %

Sample type

Tissue Extracts

Average %

= 108

Range

101 - 111 %

Sample type

Heparin Plasma

Average %

= 75

Range

70 - 82 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Tau ELISA kit (ab273617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tau protein in serum, plasma, cerebrospinal fluid, cell culture supernatant and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tau with 16 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

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3 product images

  • Sandwich ELISA - Human Tau ELISA Kit (ab273617), expandable thumbnail

    Sandwich ELISA - Human Tau ELISA Kit (ab273617)

    Human Tau standard curve comparison.

    Standard Curve comparison between the original Human Tau SimpleStep ELISA kit (ab210972) and current Human Tau SimpleStep ELISA kit (ab273617).

  • Sandwich ELISA - Human Tau ELISA Kit (ab273617), expandable thumbnail

    Sandwich ELISA - Human Tau ELISA Kit (ab273617)

    Example of human Tau standard curve in Sample Diluent NS.

    The Tau standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Tau ELISA Kit (ab273617), expandable thumbnail

    Sandwich ELISA - Human Tau ELISA Kit (ab273617)

    Interpolated concentrations of native Tau in human SH-SY5Y cell treated for 4 hours based on 80 μg/mL extract loads and human brain tissue based on a 3.5 µg/mL extract load.

    The concentrations of Tau were measured in duplicate and interpolated from the Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 2,500 pg/mL in SH-SY5Y mock treated extract, 2,233 pg/mL in SH-SY5Y staurosporine treated extract and 1,849 pg/mL in human brain extract. Staurosporine treatment induces Tau cleavage. As expected, there is minimum difference in Tau concentration between the staurosporine treated and mock-treated SH-SY5Y cell extract, as this kit does not differentiate between the full-length and cleaved form of Tau protein. The cleaved Tau fragment can be specifically measured using Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) ab269557, Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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