AB273617

Human Tau ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human Tau ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tau with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images

ELISA

Supplier Data

ELISA - Human Tau ELISA Kit (AB273617)

sELISA

Supplier Data

Sandwich ELISA - Human Tau ELISA Kit (AB273617)

Example of human Tau standard curve in Sample Diluent NS.

The Tau standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Tau ELISA Kit (AB273617)

Interpolated concentrations of native Tau in human SH-SY5Y cell treated for 4 hours based on 80 μg/mL extract loads and human brain tissue based on a 3.5 µg/mL extract load.

The concentrations of Tau were measured in duplicate and interpolated from the Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 2,500 pg/mL in SH-SY5Y mock treated extract, 2,233 pg/mL in SH-SY5Y staurosporine treated extract and 1,849 pg/mL in human brain extract. Staurosporine treatment induces Tau cleavage. As expected, there is minimum difference in Tau concentration between the staurosporine treated and mock-treated SH-SY5Y cell extract, as this kit does not differentiate between the full-length and cleaved form of Tau protein. The cleaved Tau fragment can be specifically measured using ab269557, Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713).

sELISA

Supplier Data

Sandwich ELISA - Human Tau ELISA Kit (AB273617)

Human Tau standard curve comparison.

Standard Curve comparison between the original Human Tau SimpleStep ELISA kit (ab210972) and current Human Tau SimpleStep ELISA kit (ab273617).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16 pg/mL

Range

40.63 - 2600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human Tau ELISA Kit ab273617 is a rapid single-wash 90-min ELISA kit to measure Human Tau in cerebral spinal fluid (CSF) cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 16 pg/mL.

How the assay works

Human Tau SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Tau protein in serum, plasma (EDTA and heparin), cell culture supernatant, cerebrospinal fluid, cell and tissue extract samples only. Urine, milk, saliva samples have not been tested with this kit. This kit is incompatible with plasma (citrate) samples.
The kit is 100% reactive with human recombinant Tau-A isoform and 61% reactive with human recombinant Tau-F isoform.
Recombinant human UCHL-1 and alpha synuclein were prepared at 7 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant human UCHL-1 and alpha synuclein were prepared at 50 ng/mL and 2 ng/mL and tested for interference with 2 ng/mL of recombinant human Tau. No interference with was observed.

Human Tau ELISA Kit ab273617 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Tau ELISA kit ab237617

Human Tau ELISA kit ab237617 has been used in the study of microglial surveillance in neurodegenerative diseases ⁽¹⁾.

Reference:

⁽¹⁾Dayuan Wang et al. 2022, PMID:36873190

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Mouse Tau Antibody Pair - BSA and Azide free ab253413
- Anti-Tau antibody [EPR22524-211] - BSA and Azide free (Capture) ab259552
- Anti-Tau antibody [EPR22521-17] - BSA and Azide free (Detector) ab259658

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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Recovery

[ { "sample": "Serum", "range": "76 - 84 %", "average": "= 79" }, { "sample": "EDTA Plasma", "range": "75 - 83 %", "average": "= 78" }, { "sample": "Cell culture extracts", "range": "98 - 101 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "84 - 91 %", "average": "= 86" }, { "sample": "Tissue Extracts", "range": "101 - 111 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "70 - 82 %", "average": "= 75" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273617-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB273617-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB273617-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB273617-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB273617-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB273617-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Tau Capture Antibody", "number":"AB273617-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB273617-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Tau Lyophilized Recombinant Protein", "number":"AB273617-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human Tau Detector Antibody", "number":"AB273617-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB273617-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB273617-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 13:834-851 PubMed36873190

2023

Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Dayuan Wang,Xiao Gu,Xinyi Ma,Jun Chen,Qizhi Zhang,Zhihua Yu,Juan Li,Meng Hu,Xiaofang Tan,Yuyun Tang,Jianrong Xu,Minjun Xu,Qingxiang Song,Huahua Song,Gan Jiang,Zaiming Tang,Xiaoling Gao,Hongzhuan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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