Human Tau ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Tau with a sensitivity of 2.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.08 - 20800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
What's included?
Recommended products
Human Tau ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Tau with a sensitivity of 2.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Fluorescent
- Sample types
- Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.08 - 20800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.9 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 5.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 79 | Range 76 - 84 % |
Sample type EDTA Plasma | Average % = 78 | Range 75 - 83 % |
Sample type Cerebral Spinal Fluid | Average % = 90 | Range 89 - 90 % |
Sample type Tissue Extracts | Average % = 108 | Range 101 - 111 % |
Sample type Heparin Plasma | Average % = 75 | Range 70 - 82 % |
Sample type Cell Lysate | Average % = 97 | Range 94 - 101 % |
Sample type Cell culture media | Average % = 86 | Range 84 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Tau in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tau protein in human serum, plasmas, cerebrospinal fluid, and cell and tissue extracts.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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2 product images
Sandwich ELISA - Human Tau ELISA Kit, Fluorescent (ab229394)
Example of human Tau standard curve in Sample Diluent NS.
The Tau standard curve was prepared as described in Section 10.
Sandwich ELISA - Human Tau ELISA Kit, Fluorescent (ab229394)
Interpolated concentrations of native Tau in human SH-SY5Y cell treated with or without 1 µM staurosporine.
The concentrations of Tau were measured in duplicate and interpolated from the Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 2,500 pg/mL in SH-SY5Y mock treated extract, 2,233 pg/mL in SH-SY5Y staurosporine treated extract and 1,849 pg/mL in human brain extract. Staurosporine treatment induces Tau cleavage. Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) ab269557 , the cleaved Tau fragment can be specifically measured using Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713) ab269557, Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713).
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