Human Tau ELISA Kit, Fluorescent
- CatchPoint SimpleStep ELISA
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human Tau ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Tau with a sensitivity of 2.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
View Alternative Names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
- ELISA
Supplier Data
ELISA - Human Tau ELISA Kit, Fluorescent (AB229394)
- sELISA
Unknown
Sandwich ELISA - Human Tau ELISA Kit, Fluorescent (AB229394)
Example of human Tau standard curve in Sample Diluent NS.
The Tau standard curve was prepared as described in Section 10.
- sELISA
Unknown
Sandwich ELISA - Human Tau ELISA Kit, Fluorescent (AB229394)
Interpolated concentrations of native Tau in human SH-SY5Y cell treated with or without 1 µM staurosporine.
The concentrations of Tau were measured in duplicate and interpolated from the Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 2,500 pg/mL in SH-SY5Y mock treated extract, 2,233 pg/mL in SH-SY5Y staurosporine treated extract and 1,849 pg/mL in human brain extract. Staurosporine treatment induces Tau cleavage. ab269557 , the cleaved Tau fragment can be specifically measured using ab269557, Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713).
Reactivity data
Product details
Human Tau ELISA Kit, Fluorescent ab229394 is a rapid single-wash 90-min sandwich ELISA to measure Human Tau in serum, heparin plasma, EDTA plasma, cerebral spinal fluid (CSF), cell culture extracts, cell lysate, tissue extracts. This CatchPoint SimpleStep sensitivity is 2.9 pg/mL.
How the assay works
Human Tau CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission. This kit is optimised for Molecular Devices Readers: If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Tau ELISA Kit, Fluorescent ab229394 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a fluorescent plate reader at 530/570/590 nm Excitation/Cutoff/Emission.
Colorimetric version
This kit is also available with a colorimetric readout as Human Tau ELISA Kit ab273617.
Both the fluroescent and colorimetric version share the same antibody pair and protein standard.
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Anti-Tau antibody [EPR22524-211] - BSA and Azide free (Capture) ab259552
- Anti-Tau antibody [EPR22521-17] - BSA and Azide free (Detector) ab259658.
Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com