AB325894

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular

Recombinant
SimpleStep Ignite™ ELISA
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Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Tau (phospho S396) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.12 - 9000 pg/ml with a sensitivity of 2.27 pg/mL.
- Validated on a number of sample types including cerebrospinal fluid (CSF).

- Patent pending

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

7 Images

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Dynamic range comparison between the Chemiluminescent Human Tau (phospho S396) SimpleStep ELISA, colorimetric Human Tau (phospho S396) SimpleStep ELISA (ab324328), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased range.

sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Standard curve comparison between the Chemiluminescent Human Tau (phospho S396) SimpleStep ELISA and colorimetric Human Tau (phospho S396) SimpleStep ELISA (ab324328) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tau (phospho S396) concentration of detected samples was determined to be 125.5 pg/mL with a range of undetected – 125.5 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Interpolated concentrations of native (A) human Tau (phospho S396) of 6 individual post-mortem human CSF donors measured in singlet and (B) human Tau (total, measured using ab273617) of the same 6 individual post-mortem human CSF donors measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pT217, Tau pT231 and Tau pS199) were tested for specificity (each at 25,600 pg/mL), and for interference (each at 12,800 pg/mL) combined with 1,600 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated S396 (Tau pS396). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pS396 control (for interference), are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Interpolated concentration of native Tau (phospho S396) was measured in duplicate at different sample concentrations. Undiluted samples are 50% pooled post-mortem CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.27 pg/mL

Range

4.12 - 9000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (ab325894) is a rapid single-wash sandwich 55-min ELISA to measure Human Tau (phospho S396) in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 2.27 pg/mL.

How the assay works

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (ab325894) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Tau (phospho S396) (ab325894) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.1" } ]

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Recovery

[ { "sample": "Serum", "range": "78 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "90 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "82 - 89 %", "average": "= 87" }, { "sample": "Cell culture supernatant", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Cerebral Spinal Fluid", "range": "87 - 95 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S396

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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