AB318935

Human Tau (phospho T217) ELISA Kit - Intracellular

Recombinant
SimpleStep
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Human Tau (phospho T217) ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human Tau (phospho T217) with a sensitivity of 0.09 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Interpolated concentrations of native(A) human Tau (phospho T217) and (B) human Tau (total) in normal and Alzheimer’s disease (AD) brain tissue extract samples. Interpolated concentrations of (A) human Tau (phospho T217) and (B) human Tau (total, measured using ab273617) were measured in duplicate at different sample concentrations of normal and post-mortem, individual Alzheimer’s disease (AD) human brain extract samples. The interpolated dilution factor corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

100 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT231 and Tau pS396) were tested for specificity, and for interference with 10 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T217 (Tau pT217). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pT217 control (for interference), are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Interpolated concentrations of spiked human Tau (phospho T217) in human brain cortex tissue extract in 96-well vs. 384-well plates. Recombinant Tau (phospho T217) was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plates. Undiluted samples are 15 µg/mL human brain cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Interpolated concentrations of human Tau (phospho T217) in SH-SY5Y cell extract and human Alzheimer’s disease (AD) brain hippocampus tissue extract samples. Interpolated concentration of native Tau (phospho T217) was measured in duplicate at different sample concentrations. Undiluted samples are 60 µg/mL SH-SY5Y cell extract and 12.5 µg/mL Alzheimer’s disease (AD) brain hippocampus tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Interpolated concentrations of Tau (phospho T217) and Tau (total) in normal human, mouse, and rat samples. Interpolated concentrations of (A) Tau (phospho T217) and (B) Tau (total, measured using ab273617) were measured in duplicate at a single concentration (A : 15 µg/mL and B : 0.75 µg/mL) of normal human, mouse, and rat brain tissue extract samples. The interpolated values, normalized per µg extract, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Tau (phospho T217) and Tau (total) in lambda phosphatase and mock treated mouse brain extract. Tau (phospho T217) and Tau (total, measured using ab273617) were measured in duplicate of lambda phosphatase treated (NEB P0753, 48,000 U/mL for 15 min) and mock treated mouse brain tissue extract samples. The raw O.D. values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Example of human Tau (phospho T217) standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Example of human Tau (phospho T217) standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Intracellular (AB318935)

Example of human Tau (phospho T217) standard curve in 1X Cell Extraction Buffer PTR. Example of human Tau (phospho T217) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human, Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.09 ng/mL

Range

0.469 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tau (phospho T217) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T217) protein in Cell Lysate, Tissue extracts. Quantitate Human Tau (phospho T217) with 0.09 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "90 - 106 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "91 - 103 %", "average": "= 97" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho T217

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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