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Human Tau (phospho T217) ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (phospho T217) - Intracellular in Cell Lysate, Tissue Extracts samples.

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Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human, Mouse, Rat

Range

0.469 - 30 ng/mL

Assay time

1h 30m

Sensitivity

= 0.09 ng/mL

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Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

10 x 96 Tests
Components
10X Human Tau (phospho T217) Capture Antibody
10 x 600 µL
10X Human Tau (phospho T217) Detector Antibody
10 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
5X Cell Extraction Buffer PTR (ab193970)
2 x 50 mL
Antibody Diluent CPR2
10 x 6 mL
Human Tau (phospho T217) Lyophilized Recombinant Protein
10 x 2 Vial
Plate Seal
10 x 1 Unit
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 96 Wells
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

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Human Tau (phospho T217) ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (phospho T217) - Intracellular in Cell Lysate, Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human, Mouse, Rat

Range

0.469 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.09 ng/mL

General recovery

97%

Precision

Intra assay

Sample
Extract
n
8
mean
-
SD
-
C.V.
7.2

Inter assay

Sample
Extract
n
3
mean
-
SD
-
C.V.
5

Recovery

Sample specific recovery

Sample type
Tissue Extracts
Average %
= 96
Range
90 - 106 %
Sample type
Cell culture extracts
Average %
= 97
Range
91 - 103 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Tau (phospho T217) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T217) protein in Cell Lysate, Tissue extracts. Quantitate Human Tau (phospho T217) with 0.09 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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