Human Tau (phospho T231) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (phospho T231) - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 87.5 - 5600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 34 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
What's included?
Human Tau (phospho T231) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tau (phospho T231) - Extracellular in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 87.5 - 5600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 34 pg/mL
- General recovery
- 90%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 4.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 2.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 80 - 98 % |
Sample type Citrate plasma | Average % = 86 | Range 82 - 88 % |
Sample type EDTA Plasma | Average % = 86 | Range 85 - 87 % |
Sample type Heparin Plasma | Average % = 86 | Range 84 - 88 % |
Sample type Cell culture media | Average % = 88 | Range 84 - 93 % |
Sample type Cerebral Spinal Fluid | Average % = 99 | Range 95 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tau (phospho T231) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T231) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human Tau (phospho T231) with 34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
30 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT217 and Tau pS396) were tested for specificity and for interference with 3 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T231 (Tau pT231). The interpolated dilution-corrected values, expressed as % of values of Tau pT231, are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations. Undiluted samples are 50% CSF from a single, post-mortem female donor. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Recombinant Tau (phospho T231) was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Recombinant Tau (phospho T231) was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS in 96-well vs. 384-well plates. Undiluted samples are 12.5% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Example of human Tau (phospho T231) standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Recombinant Tau (phospho T231) was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows: cell culture media 100%, and CSF 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367)
Example of human Tau (phospho T231) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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