AB322367

Human Tau (phospho T231) ELISA Kit- Extracellular

Name: Human Tau (phospho T231) ELISA Kit- Extracellular (ab322367) | Abcam
Brand: Abcam Limited
SKU: AB322367
Price: 705 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

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Human Tau (phospho T231) ELISA Kit- Extracellular is a Sandwich (quantitative) ELISA for the measurement of Human Tau (phospho T231) - Extracellular in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

7 Images

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations. Undiluted samples are 50% CSF from a single, post-mortem female donor. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

30 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT217 and Tau pS396) were tested for specificity and for interference with 3 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T231 (Tau pT231). The interpolated dilution-corrected values, expressed as % of values of Tau pT231, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Recombinant Tau (phospho T231) was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS in 96-well vs. 384-well plates. Undiluted samples are 12.5% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Recombinant Tau (phospho T231) was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : cell culture media 100%, and CSF 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Recombinant Tau (phospho T231) was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Example of human Tau (phospho T231) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Extracellular (AB322367)

Example of human Tau (phospho T231) standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 34 pg/mL

Range

87.5 - 5600 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tau (phospho T231) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T231) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human Tau (phospho T231) with 34 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "82 - 88 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "84 - 88 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "84 - 93 %", "average": "= 88" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 101 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho T231

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

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