AB322366

Human Tau (phospho T231) ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human Tau (phospho T231) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tau (phospho T231) with a sensitivity of 21 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

8 Images

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

30 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT217 and Tau pS396) were tested for specificity and for interference with 3 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T231 (Tau pT231). The interpolated dilution-corrected values, expressed as % of values of Tau pT231, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Interpolated concentrations of (A) Tau (phospho T231) and (B) total Tau (ab273617) were measured in duplicate at various concentrations of normal human, mouse, and rat brain tissue extract samples. The interpolated dilution-corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Interpolated concentrations of (A) human Tau (phospho T231) and (B) human total Tau (ab273617) were measured in duplicate at different sample concentrations of normal and Alzheimer’s disease (AD) post-mortem human brain extract samples. The interpolated dilution factor corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2). For comparison of Tau phosphorylation at alternate site in identical AD brain tissue samples, see the following ELISA kit product : ab318935 (Tau pT217).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations. Undiluted samples are as follows : SH-SY5Y cell extract 25 µg/mL, human brain cortex tissue extract 12 µg/mL, and human brain hippocampus tissue extract 15 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1.5 µg/mL brain cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Tau (phospho T231) and total Tau (ab273617) were measured in duplicate of lambda phosphatase treated (NEB P0753, 48,000 U/mL for 15 min) and mock treated mouse brain tissue extract samples. The raw O.D. values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Example of human Tau (phospho T231) standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (AB322366)

Example of human Tau (phospho T231) standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tau (phospho T231) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T231) protein in Cell Lysate, Tissue extracts. Quantitate Human Tau (phospho T231) with 21 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "102 - 119 %", "average": "= 112" }, { "sample": "Tissue Extracts", "range": "115 - 118 %", "average": "= 116" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho T231

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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