Human Tau (phospho T231) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tau (phospho T231) with a sensitivity of 21 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 21 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
What's included?
Human Tau (phospho T231) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Tau (phospho T231) with a sensitivity of 21 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 21 pg/mL
- General recovery
- 112%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 4.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 6.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 112 | Range 102 - 119 % |
Sample type Tissue Extracts | Average % = 116 | Range 115 - 118 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tau (phospho T231) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho T231) protein in Cell Lysate, Tissue extracts. Quantitate Human Tau (phospho T231) with 21 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Interpolated concentrations of (A) Tau (phospho T231) and (B) total Tau (Human Tau ELISA Kit ab273617) were measured in duplicate at various concentrations of normal human, mouse, and rat brain tissue extract samples. The interpolated dilution-corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Tau (phospho T231) and total Tau (Human Tau ELISA Kit ab273617) were measured in duplicate of lambda phosphatase treated (NEB P0753, 48,000 U/mL for 15 min) and mock treated mouse brain tissue extract samples. The raw O.D. values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Interpolated concentrations of (A) human Tau (phospho T231) and (B) human total Tau (Human Tau ELISA Kit ab273617) were measured in duplicate at different sample concentrations of normal and Alzheimer’s disease (AD) post-mortem human brain extract samples. The interpolated dilution factor corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2). For comparison of Tau phosphorylation at alternate site in identical AD brain tissue samples, see the following ELISA kit product: Human Tau (phospho T217) ELISA Kit - Intracellular ab318935 (Tau pT217).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations. Undiluted samples are as follows: SH-SY5Y cell extract 25 µg/mL, human brain cortex tissue extract 12 µg/mL, and human brain hippocampus tissue extract 15 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Interpolated concentration of native Tau (phospho T231) was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1.5 µg/mL brain cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Example of human Tau (phospho T231) standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
30 ng/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pS199, Tau pT217 and Tau pS396) were tested for specificity and for interference with 3 ng/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T231 (Tau pT231). The interpolated dilution-corrected values, expressed as % of values of Tau pT231, are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Tau (phospho T231) ELISA Kit- Intracellular (ab322366)
Example of human Tau (phospho T231) standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com