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AB314701

Human TAZ ELISA Kit

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Human TAZ ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TAZ in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

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Sandwich ELISA - Human TAZ ELISA Kit (AB314701)
  • sELISA

Supplier Data

Sandwich ELISA - Human TAZ ELISA Kit (AB314701)

Example of standard curve using ab314701. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.3 ng/mL

Range

1.3 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TAZ ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TAZ in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human TAZ coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "91 - 136 %", "average": "= 115.6" }, { "sample": "Plasma", "range": "91 - 138 %", "average": "= 112.5" }, { "sample": "Cell culture media", "range": "123 - 136 %", "average": "= 126.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314701-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314701-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314701-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314701-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TAZ Protein Standard", "number":"AB314701-CMP11", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314701-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TAZ Antibody-coated ELISA Plate", "number":"AB314701-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TAZ Detection Antibody", "number":"AB314701-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314701-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB314701-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.
Biological function summary

TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.

Pathways

TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.

TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.
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Product protocols

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Target data

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 11118213, PubMed : 18227151, PubMed : 23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed : 19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed : 18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed : 18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed : 18227151, PubMed : 18568018).
See full target information WWTR1
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