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AB213759

Human TCblR ELISA Kit (8D6A)

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(1 Publication)

Human TCblR ELISA Kit (8D6A) is a Sandwich ELISA for the measurement of Human TCblR (8D6A) in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD320, 8D6A, UNQ198/PRO224, CD320 antigen, 8D6 antigen, FDC-signaling molecule 8D6, Transcobalamin receptor, FDC-SM-8D6, TCblR

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Sandwich ELISA - Human TCblR ELISA Kit (8D6A) (AB213759)
  • sELISA

Supplier Data

Sandwich ELISA - Human TCblR ELISA Kit (8D6A) (AB213759)

Human TCblR ELISA Kit (8D6A) (ab213759) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB276989

Recombinant mouse TCblR protein (His tag)

Functional Studies - Recombinant mouse TCblR protein (His tag) (AB276989)

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Reagents, Controls & Accessories

AB171523

TMB ELISA Substrate (High Sensitivity)

ELISA - TMB ELISA Substrate (High Sensitivity) (AB171523)

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Reagents, Controls & Accessories

AB171529

450 nm Stop Solution for TMB Substrate

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Reagents, Controls & Accessories

AB171522

TMB ELISA Substrate (Highest Sensitivity)

ELISA - TMB ELISA Substrate (Highest Sensitivity) (AB171522)

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Reactivity data

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Product details

The Human TCblR Enzyme-Linked Immunosorbent Assay (ELISA) kit (8D6A) (ab213759) is designed for the quantitative measurement of Human TCblR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for TCblR has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TCblR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TCblR amount of sample captured in plate.

TCblR (cluster of differentiation 320), also known as 8D6A or CD320, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of TCblR enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the TCblR signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. TCblR is also a specific receptor for uptake of TCN2-bound cobalamin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1.142 ng/mL", "standardDeviation": "0.081", "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "5.029 ng/mL", "standardDeviation": "0.326", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1.266 ng/mL", "standardDeviation": "0.072", "coefficientOfVariability": "= 5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "0.322 ng/mL", "standardDeviation": "0.017", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.142 ng/mL", "standardDeviation": "0.014", "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4.609 ng/mL", "standardDeviation": "0.271", "coefficientOfVariability": "= 5.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcobalamin receptor (TCblR) also known as CD320 is a membrane protein involved in the uptake of vitamin B12 (cobalamin) by cells. TCblR is recognized for its role in the binding and internalization of transcobalamin-bound vitamin B12 enabling this essential vitamin to enter cells. The protein has a molecular mass of approximately 50 kDa. TCblR is expressed on the surface of many cell types with notable expression in bone marrow lymphoid tissues and certain tumor cells.
Biological function summary

TCblR facilitates the cellular uptake of vitamin B12 which is necessary for DNA synthesis and cell growth. It is not part of a larger protein complex but it interacts directly with transcobalamin a carrier protein that escorts vitamin B12 through the bloodstream. The interaction between TCblR and transcobalamin-bound vitamin B12 permits the transport of this vitamin into the cell where it plays a role in metabolic processes.

Pathways

TCblR plays an important role in the cobalamin metabolism pathway by mediating the internalization of vitamin B12 into cells. This uptake is important for the methionine and folate cycles where vitamin B12 functions as a cofactor. TCblR is therefore linked to the methionine synthase enzyme which is part of the folate cycle and necessary for the conversion of homocysteine to methionine.

Mutations or deficiencies in TCblR function can lead to conditions like megaloblastic anemia and neuropathy due to vitamin B12 deficiency. Altered expression of TCblR has been associated with certain types of cancer where it can influence cell proliferation by modulating vitamin B12 uptake. Its interaction with the transcobalamin protein highlights its relevance in maintaining B12 levels and cellular health.
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Product protocols

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Target data

Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed : 18779389). Plays an important role in cobalamin uptake (PubMed : 18779389, PubMed : 20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed : 10727470). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed : 10727470, PubMed : 11418631). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed : 11418631). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed : 11418631).
See full target information CD320
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federa 32:5506-5519 PubMed29741927

2018

Transcellular transport of cobalamin in aortic endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luciana Hannibal,Keerthana Bolisetty,Armend Axhemi,Patricia M DiBello,Edward V Quadros,Sergey Fedosov,Donald W Jacobsen
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