Human TCF-4/TCF7L2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TCF-4/TCF7L2 with a sensitivity of 49.873 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 49.873 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Alternative names
TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4
What's included?
Recommended products
Human TCF-4/TCF7L2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TCF-4/TCF7L2 with a sensitivity of 49.873 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 49.873 pg/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 11.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 11.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 93 | Range 82 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TCF-4/TCF7L2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TCF-4/TCF7L2 protein in human cell lysate. Quantitate Human TCF-4/TCF7L2 with 49.873 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF-4 also known as TCF7L2 is a transcription factor that plays an important mechanical role in the Wnt signaling pathway. It is a protein with a molecular mass of approximately 66 kDa and is expressed in many tissues including the intestine pancreas and brain. TCF-4 acts by binding to DNA at specific sites and regulating the transcription of target genes. This transcription regulation occurs when TCF-4 partners with beta-catenin altering gene expression in response to Wnt signals.
- Biological function summary
TCF-4 influences processes like cell proliferation differentiation and apoptosis. It functions as part of a transcriptional complex with members such as beta-catenin and other cofactors which are essential for its regulatory activities. Through its control of gene expression TCF-4 affects tissue homeostasis and has implications in embryonic development. It modulates the expression of genes that are involved in important cellular processes making it a significant player in maintaining cellular equilibrium.
- Pathways
TCF-4 is essential in the Wnt/beta-catenin signaling pathway a critical pathway that regulates cell fate and patterning during development. This pathway involves the accumulation of beta-catenin in the cytoplasm and its subsequent translocation into the nucleus. In the nucleus beta-catenin partners with TCF-4 to regulate gene transcription. Additionally TCF-4 interacts with other pathway proteins such as LEF1 (Lymphoid Enhancer-Binding Factor 1) to modulate Wnt target genes illustrating its integration within complex signaling networks.
- Associated diseases and disorders
TCF-4 has strong connections to type 2 diabetes and colorectal cancer. Variations in the TCF7L2 gene have been linked to increased risk of type 2 diabetes potentially due to its role in glucose metabolism. In colorectal cancer aberrant activation of the Wnt signaling pathway often involving TCF-4 and beta-catenin leads to uncontrolled cell growth. Understanding these connections can provide insights into the pathogenesis of these diseases and inform treatment strategies targeting TCF-4 and its associated pathways.
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2 product images
Sandwich ELISA - Human TCF-4/TCF7L2 ELISA Kit (ab309185)
Interpolated concentration of native TCF-4/TCF7L2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 1000 μg/mL HCT 116 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human TCF-4/TCF7L2 ELISA Kit (ab309185)
Example of human TCF-4/TCF7L2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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