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AB283974

Human TDP1 ELISA Kit

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Human TDP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TDP1 in Human in Cell Culture Media samples.

View Alternative Names

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1

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Sandwich ELISA - Human TDP1 ELISA Kit (AB283974)
  • sELISA

Supplier Data

Sandwich ELISA - Human TDP1 ELISA Kit (AB283974)

Example of Human TDP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1 ng/mL

Range

2.563 - 100 µg/mL

Assay time

5h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human TDP1 ELISA Kit is designed for detection of TDP1 in human cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human TDP1 in approximately 5 hours.

A polyclonal antibody specific for human TDP1 has been pre-coated onto a 96-well microplate with removable strips. TDP1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TDP1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP1 short for Tyrosyl-DNA Phosphodiesterase 1 is an enzyme involved in the repair of DNA damage. Also known as SCAN1 protein this enzyme has a mass of approximately 60 kDa and is expressed in a variety of tissues with higher levels seen in the brain and heart. It plays a mechanical role by hydrolyzing the phosphodiester bond between a DNA 3'-phosphate and a tyrosine residue an important step in resolving topoisomerase I-DNA crosslinks during the repair of stalled replication forks.
Biological function summary

TDP1 contributes significantly to the maintenance of genomic stability. It functions primarily as a DNA repair enzyme acting independently rather than as part of a protein complex. TDP1's ability to cleave phosphotyrosyl bonds makes it integral to processes that safeguard against DNA strand breaks induced by oxidative stress or other exogenous factors. Though not part of a complex its function is supported by the interaction with proteins like DNA polymerase which aids in the subsequent steps of DNA repair synthesis.

Pathways

TDP1 is involved in the DNA damage response and repair pathways. It collaborates heavily with the Base Excision Repair (BER) and the Single-Strand Break Repair (SSBR) pathways. TDP1 works alongside proteins like PARP1 facilitating the repair of single-strand DNA breaks which is critical for cell survival and prevention of mutations. Aphidicolin a known inhibitor of DNA polymerase can affect DNA synthesis steps that follow TDP1 activity indicating how interconnected these pathways are.

TDP1 has notable associations with certain neurodegenerative diseases and cancer. Mutations or dysregulation in TDP1 have been implicated in the autosomal recessive disorder known as Spinocerebellar Ataxia with Axonal Neuropathy (SCAN1). Additionally due to its role in DNA repair TDP1 may influence cancer progression as cells with impaired DNA repair capabilities accumulate mutations that could lead to tumorigenesis. The interaction with methotrexate a chemotherapeutic agent can also affect cancer treatment outcomes given methotrexate's influence on folate metabolism and DNA synthesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
See full target information TDP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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