JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282880

Human TDP43 ELISA Kit (TARDBP)

Be the first to review this product! Submit a review

|

(0 Publication)

Human TDP43 ELISA Kit (TARDBP) is a single-wash 90-min Simplestep used to quantify Human TDP43 (TARDBP) with a sensitivity of 248.24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43

4 Images
ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)
  • ELISA

Supplier Data

ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)
Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)
  • sELISA

Supplier Data

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)

Representative image of Human TDP43 ELISA Kit (TARDBP) (ab282880)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human TDP43 (TARDBP) Capture Antibody

- 10X Human TDP43 (TARDBP) Detector Antibody

- Human TDP43 (TARDBP) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)
  • sELISA

Supplier Data

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)

Interpolated concentrations of native TDP43 in human K562 extract, OVCAR-3 extract, and human skeletal muscle extract based on a 20 μg/mL extract load for K562 extract, 50 μg/mL extract load for OVCAR-3 extract, and 100 μg/mL extract load for skeletal muscle extract. The concentrations of TDP43 were measured in duplicate and interpolated from the TDP43 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TDP43 concentration was determined to be 70.6 ng/mL in K562 extract, 89.24 ng/mL in OVCAR-3 extract and 85.05 ng/mL in skeletal muscle extract.

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)
  • sELISA

Supplier Data

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880)

Example of human TDP43 standard curve in 1X Cell Extraction Buffer PTR.

The TDP43 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 248.24 pg/mL

Range

1171.87 - 75000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

Human TDP43 ELISA Kit (TARDBP) ab282880 is a rapid single-wash 90-min Sandwich ELISA to measure Human TDP43 (TARDBP)in cell lysate, tissue extracts. This SimpleStep sensitivity is 248.24 pg/mL.

How the assay works

Human TDP43 (TARDBP)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TDP43 ELISA Kit (TARDBP) ab282880 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "108 - 115 %", "average": "= 111" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282880-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282880-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282880-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB282880-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282880-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282880-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282880-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TDP43 (TARDBP) Capture Antibody", "number":"AB282880-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TDP43 (TARDBP) Lyophilized Recombinant Protein", "number":"AB282880-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB282880-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282880-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282880-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TDP43 (TARDBP) Detector Antibody", "number":"AB282880-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
Biological function summary

TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

Pathways

Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
style
left-column

Product protocols

style
left-column

Target data

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed : 23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed : 23519609, PubMed : 24240615, PubMed : 24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed : 21358640, PubMed : 29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed : 28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed : 30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed : 19765185, PubMed : 23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed : 30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed : 27123980). Negatively regulates the expression of CDK6 (PubMed : 19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed : 25678563).
See full target information TARDBP
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com