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Human TDP43 ELISA Kit (TARDBP) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TDP43 (TARDBP) in Tissue Extracts, Cell Lysate samples.

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Images

Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880), expandable thumbnail
  • Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (AB282880), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1171.87 - 75000 pg/mL

Assay time

1h 30m

Sensitivity

= 248.24 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:19765185, PubMed:23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human TDP43 (TARDBP) Capture Antibody
1 x 600 µL
10X Human TDP43 (TARDBP) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 5BI
1 x 6 mL
Human TDP43 (TARDBP) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human TDP43 ELISA Kit (TARDBP) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human TDP43 (TARDBP) in Tissue Extracts, Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1171.87 - 75000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 248.24 pg/mL

Precision

Intra assay

Sample

Extract

n
8
mean
-
SD
-
C.V.

6.2

Inter assay

Sample

Extract

n
3
mean
-
SD
-
C.V.

7.8

Recovery

Sample specific recovery

Sample type

Cell Lysate

Average %

= 111

Range

108 - 115 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human TDR43 ELISA kit (ab282880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TDR43 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TDR43 with 248.24 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human TDP43 is an RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, human TDP43 regulates the splicing of many non-coding and protein coding RNAs including proteins involved in neuronal survival and mRNAs that encode proteins relevant for neurodegenerative diseases. Human TDP43 plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Human TDP43 regulates mRNA. It also participates in normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. It is involved with regulation of expression of CDK6 and HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.

Biological function summary

TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

Pathways

Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

Associated diseases and disorders

The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.

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2 product images

  • Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (ab282880), expandable thumbnail

    Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (ab282880)

    Example of human TDP43 standard curve in 1X Cell Extraction Buffer PTR.

    The TDP43 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (ab282880), expandable thumbnail

    Sandwich ELISA - Human TDP43 ELISA Kit (TARDBP) (ab282880)

    Interpolated concentrations of native TDP43 in human K562 extract, OVCAR-3 extract, and human skeletal muscle extract based on a 20 μg/mL extract load for K562 extract, 50 μg/mL extract load for OVCAR-3 extract, and 100 μg/mL extract load for skeletal muscle extract. The concentrations of TDP43 were measured in duplicate and interpolated from the TDP43 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TDP43 concentration was determined to be 70.6 ng/mL in K562 extract, 89.24 ng/mL in OVCAR-3 extract and 85.05 ng/mL in skeletal muscle extract.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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