AB277081

Human Tenascin C ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human Tenascin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tenascin C with a sensitivity of 27.672 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HXB, TNC, Tenascin, TN, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, TN-C

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Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Interpolated concentrations of native Tenascin C in human serum and plasma samples.

The concentrations of Tenascin C were measured in duplicates, interpolated from the Tenascin C standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 153.25 ng/mL in serum, 113.37 ng/mL in plasma (citrate), 108.74 ng/mL in plasma (EDTA), and 101.20 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

5% serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tenascin C concentration was determined to be 351.07 ng/mL with a range of 247.61 – 487.97 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Example of human Tenascin C standard curve in Sample Diluent NS. The Tenascin C standard curve was prepared as described in Section 10.

The Tenascin C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 27.672 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Tenascin C ELISA kit (ab277081) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tenascin C protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tenascin C with 27.67 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tenascin C (TNC), is an extracellular matrix glycoprotein involved in tissue morphogenesis like embryogenesis, wound healing and tumorigenesis. Tenascin C is implicated in guidance of migrating neurons and axons during development, synaptic plasticity and neuronal regeneration. Tenascin C also as a role in neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. In tumors, Tenascin C stimulates angiogenesis by elongation, migration and sprouting of endothelial cell. Tenascin C shares 69.0%, 70.6%, 72.0%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 106 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 99 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "84 - 94 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "83 - 106 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tenascin C also known as hexabrachion is an extracellular matrix glycoprotein with a mass of approximately 220-300 kDa depending on post-translational modifications. It shows high expression in areas undergoing tissue remodeling such as embryonic tissues wounds and tumors. It consists of repeating structural elements including epidermal growth factor-like repeats and fibronectin type III domains which facilitate its function in the context of tissue dynamics.

Biological function summary

The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.

Pathways

Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.

Tenascin C exhibits a strong connection to cancer progression and chronic inflammatory conditions such as rheumatoid arthritis. Its levels rise in tumors and inflamed tissues where it interacts with other matrix components and cytokines like interleukin-6 (IL-6). These interactions contribute to a microenvironment that promotes tumor growth and persistence of inflammation highlighting its role in the pathophysiology of these diseases.

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Product protocols

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Target data

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed : 19884327).

See full target information TNC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:12028 PubMed38797735

2024

The serum tenascin C level is a marker of metabolic disorder-related inflammation affecting pancreatic cancer prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiko Sato,Hayato Hikita,Minoru Shigekawa,Kazumasa Soma,Ryohei Yamauchi,Jihyun Sung,Seiya Kato,Yoichi Sasaki,Shinnosuke Kudo,Kenji Fukumoto,Kumiko Shirai,Kazuhiro Murai,Yuki Tahata,Teppei Yoshioka,Akira Nishio,Yoshinobu Saito,Takahiro Kodama,Yutaka Sasaki,Tomohide Tatsumi,Tetsuo Takehara

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Tenascin C ELISA Kit

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Sandwich ELISA - Human Tenascin C ELISA Kit (AB277081)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise