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Human TERT ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TERT in Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Range

0.156 - 10 ng/mL

Sensitivity

< 0.094 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

Alternative names

What's included?

96 Test
Components
25X Wash buffer
1 x 30 mL
Antibody dilution buffer
1 x 10 mL
Biotin-Detection antibody (Concentrated)
1 x 120 µL
HRP-Streptavidin Conjugate (SABC)
1 x 120 µL
Lyophilized Standard
2 x 1 Vial
Micro ELISA Plate
1 x 1 Unit
Plate Sealer
5 x 1 Unit
SABC dilution buffer
1 x 10 mL
Sample and Standard Dilution Buffer
1 x 20 mL
Stop Solution
1 x 10 mL
TMB Substrate
1 x 10 mL

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Human TERT ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TERT in Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Range

0.156 - 10 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

Sensitivity

< 0.094 ng/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

This Telomerase reverse transcriptase (TERT) ELISA kit (ab285288, K4187) is a sandwich ELISA assay for the quantitative measurement of human TERT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of TERT captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called K4187 TERT (Human) ELISA Kit. K4187-100 is the same size as the 96 test size of ab285288.

Supplementary info

Biological summary

TERT plays an important role in maintaining telomere length essential for protecting chromosome stability. The protein forms part of the telomerase holoenzyme complex working together with TERC to perform its function. Telomerase activity allows cells to bypass the Hayflick limit preventing senescence and enhancing replication potential. In addition to telomere maintenance TERT influences gene regulation and cellular proliferation.

Mechanical summary

Telomerase reverse transcriptase also known as TERT is an enzyme with a molecular mass of about 127 kDa. It functions as a catalytic subunit of the telomerase complex which extends telomeres by adding the nucleotide sequence "TTAGGG" to the ends of chromosomes. TERT operates with a telomerase RNA component (TERC) to synthesize DNA using an RNA template a process called reverse transcription. TERT is expressed in embryonic tissues adult stem cells and cancer cells where rapid cell division occurs.

Pathway

TERT holds significance in the telomere maintenance and signaling pathways. Telomere maintenance ensures genomic integrity and cellular longevity a process fundamental for tissue homeostasis and regeneration. TERT interacts with proteins such as dyskerin and TERC within these pathways. Its regulatory roles in these pathways also reveal interactions with other key proteins like hypothesized targets in the DNA damage response.

Disease

The upregulation of TERT is associated with cancer and degenerative diseases. Increased telomerase activity is a common feature in many cancers leading to uncontrolled cell proliferation. Additionally mutations in TERT and its associated components can contribute to disorders like dyskeratosis congenita a rare genetic condition resulting in bone marrow failure. In these conditions TERT interaction with proteins involved in chromosomal maintenance and repair becomes important for understanding disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • ELISA - Human TERT ELISA Kit (ab285288), expandable thumbnail

    ELISA - Human TERT ELISA Kit (ab285288)

    Human TERT ELISA Kit.

    Standard curve using Human TERT ELISA Kit ab285288 where R2 = 0.9888. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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