JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB178663

Human Testosterone free ELISA Kit

Be the first to review this product! Submit a review

|

(5 Publications)

Human Testosterone free ELISA Kit is a competitive ELISA designed to quantify Human free testosterone with a sensitivity of 0.06 pg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.1 - 42  pg/mL

View Alternative Names

17b hydroxy 4 androsten 3 one, 17鑴-hydroxyandrost-4-ene-3-one

1 Images
Competitive ELISA - Human Testosterone free ELISA Kit (AB178663)
  • cELISA

Supplier Data

Competitive ELISA - Human Testosterone free ELISA Kit (AB178663)

Representative standard curve using ab178663.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

>= 0.06 pg/mL

Range

0.1 - 42 pg/mL

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 15, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178663-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"10X Wash Solution", "number":"AB178663-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178663-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Testosterone IgG Coated Microplate (12 x 8 wells)", "number":"AB178663-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178663-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178663-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 5 - 100 pg/mL", "number":"AB178663-CMP15", "productcode":"" }, { "size":"1 x 1 mL", "name":"Free Testosterone Control A", "number":"AB178663-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178663-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 4 - 20 pg/mL", "number":"AB178663-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 1 - 0.2 pg/mL", "number":"AB178663-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 2 - 1.0 pg/mL", "number":"AB178663-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"Testosterone HRP Conjugate", "number":"AB178663-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Free Testosterone Control B", "number":"AB178663-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 3 - 4.0 pg/mL", "number":"AB178663-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Testosterone Standard 0 - 0 pg/mL", "number":"AB178663-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Testosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.
Biological function summary

Testosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.

Pathways

Testosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.

Low levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.
style
left-column

Product protocols

style
left-column

Target data

See full target information Testosterone
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of applied physiology (Bethesda, Md. : 1985) 136:1388-1399 PubMed38385186

2024

Postabsorptive and postprandial myofibrillar protein synthesis rates at rest and after resistance exercise in women with postmenopause.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen F McKenna,Andrew T Askow,Kevin J M Paulussen,Amadeo F Salvador,Hsin-Yu Fang,Alexander V Ulanov,Zhong Li,Scott A Paluska,Joseph W Beals,Ralf Jäger,Martin Purpura,Nicholas A Burd

Journal of clinical medicine 11: PubMed36362788

2022

Early Changes in Androgen Levels in Individuals with Spinal Cord Injury: A Longitudinal SwiSCI Study.

Applications

Unspecified application

Species

Unspecified reactive species

Oche Adam Itodo,Peter Francis Raguindin,Jens Wöllner,Inge Eriks-Hoogland,Xavier Jordan,Margret Hund-Georgiadis,Taulant Muka,Jürgen Pannek,Jivko Stoyanov,Marija Glisic

Molecular medicine reports 22:3851-3861 PubMed32901834

2020

Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yijiao Yang,Ling Yang,Cao Qi,Guohua Hu,Longhui Wang,Zhuojun Sun,Xiaorong Ni

Journal of molecular histology 48:403-415 PubMed28988314

2017

The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement.

Applications

Unspecified application

Species

Unspecified reactive species

Flávia B Constantino,Ana C L Camargo,Sérgio A A Santos,Ketlin T Colombelli,Laura F Martin,Marcia G Silva,Sérgio L Felisbino,Luis A Justulin

General and comparative endocrinology 246:258-269 PubMed28041790

2017

Impairment of microvascular angiogenesis is associated with delay in prostatic development in rat offspring of maternal protein malnutrition.

Applications

Unspecified application

Species

Unspecified reactive species

Ketlin T Colombelli,Sérgio A A Santos,Ana C L Camargo,Flávia B Constantino,Caroline N Barquilha,Jaqueline C Rinaldi,Sérgio L Felisbino,Luis A Justulin
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB108667

17 beta Estradiol ELISA Kit

Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)

  • 4
  • 2
Assay Kits

AB179884

Human Leptin ELISA Kit

SimpleStep

ELISA - Human Leptin ELISA Kit (AB179884)

  • 0
  • 0
Assay Kits

AB108670

Human Progesterone ELISA Kit

ELISA - Human Progesterone ELISA Kit (AB108670)

  • 5
  • 1
Assay Kits

AB211651

Human IGF1 ELISA Kit

SimpleStep

ELISA - Human IGF1 ELISA Kit (AB211651)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com