Human Tetranectin ELISA Kit (CLEC3B) is a Sandwich (quantitative) ELISA kit for the measurement of Human Tetranectin (CLEC3B) in Human in Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 312 - 20000 pg/mL
- Assay time
- 3h 30m
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis. Plays a role in retinal function (PubMed:35331648).
Alternative names
TNA, CLEC3B, Tetranectin, TN, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein
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Human Tetranectin ELISA Kit (CLEC3B) is a Sandwich (quantitative) ELISA kit for the measurement of Human Tetranectin (CLEC3B) in Human in Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 312 - 20000 pg/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 3 | n 16 | mean 8208 pg/mL | SD 4632.45 | C.V. = 5.7 |
Sample Sample 2 | n 16 | mean 2683 pg/mL | SD 107.32 | C.V. = 4 |
Sample Sample 1 | n 16 | mean 427 pg/mL | SD 28.6 | C.V. = 6.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 2 | n 24 | mean 2547 pg/mL | SD 150.27 | C.V. = 5.9 |
Sample Sample 3 | n 24 | mean 7804 pg/mL | SD 561.88 | C.V. = 7.2 |
Sample Sample 1 | n 24 | mean 405 pg/mL | SD 32.4 | C.V. = 8 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Human Tetranectin Enzyme-Linked Immunosorbent Assay (ELISA) kit (CLEC3B) (ab213832) is designed for the quantitative measurement of Human Tetranectin in cell supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for Tetranectin/CLEC3B has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E22-V202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Tetranectin/CLEC3B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tetranectin/CLEC3B amount of sample captured in plate.
Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and non-covalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tetranectin also known as C-type lectin domain family 3 member B (CLEC3B) is a glycoprotein with a molecular mass of about 20 kDa. It primarily exists in the bloodstream where it acts in various physiological processes. This protein expresses itself in other tissues such as liver and lungs suggesting a broad functional role across different biological systems. Its intuitive structure and function mark it for various protein-protein interactions.
- Biological function summary
Tetranectin binds specifically to plasminogen a proenzyme which plays a role in breaking down fibrin in blood clots. It is known to be a part of the fibrinolytic system where it aids in tissue remodeling and repair by promoting the activation of plasminogen to plasmin. These activities suggest an integral role in maintaining vascular health and tissue dynamics. Moreover its involvement in these processes highlights the multifunctional attributes of this protein within human biology.
- Pathways
Tetranectin is a component within coagulation and fibrinolytic pathways. Its interaction with plasminogen highlights its importance in the conversion pathway of plasminogen to plasmin which is essential in dissolving fibrin clots. Additionally Tetranectin’s contributions in the extracellular matrix further connect it with other proteins such as fibronectin and vitronectin facilitating cellular infrastructure and wound healing.
- Associated diseases and disorders
Researchers have linked alterations in Tetranectin levels with various conditions particularly cancer and osteoporosis. In cancer it associates with tumor growth and metastasis due to its role in tissue remodeling and plasminogen activation. In osteoporosis diminished levels of Tetranectin correlate with impaired bone density and integrative functions. Its interaction with plasminogen within these diseases illustrates not only its importance in fibrinolytic pathways but also its potential as a biomarker for pathological conditions.
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SDS-PAGE - Human Tetranectin ELISA Kit (CLEC3B) (ab213832)
Human Tetranectin ELISA Kit (CLEC3B) (ab213832) Standard Curve.
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