AB277718

Human TFF1 ELISA Kit

Recombinant
SimpleStep
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Human TFF1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TFF1 with a sensitivity of 2.91 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BCEI, PS2, TFF1, Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, Protein pS2, hP1.A

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Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

sELISA

Supplier Data

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

Example of human TFF1 standard curve in Sample Diluent NS.

The TFF1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

5% Serum from ten individual healthy human female donors was measured in duplicate

sELISA

Supplier Data

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

Interpolated concentrations of native TFF1 in human serum, plasma and urine samples.

The concentrations of TFF1 were measured in duplicates, interpolated from the TFF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 6.25%, plasma (EDTA) 6.25%, plasma (heparin) 6.25%, and urine 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF1 concentration was determined to be 18.85 ng/mL in serum, 2.85 ng/mL in plasma (citrate), 2.48 ng/mL in plasma (EDTA), 2.27 ng/mL in plasma (Heparin), and 152.70 ng/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.91 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TFF1 ELISA kit (ab277704) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFF1 protein in human serum, plasma, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFF1 with 2.91 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. Trefoils are stable secretory proteins expressed in gastrointestinal mucosa. TFF1 function is not fully defined but is suggested to protect the gastric mucosa by stabilizing the mucus layer and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to nephrocalcin. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. Mouse and rat share 67 % and 69 % sequence homology to human TFF1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 94 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 99 %", "average": "= 93" }, { "sample": "Urine", "range": "97 - 105 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "87 - 89 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "80 - 90 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "95 - 99 %", "average": "= 97" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Estrogen Inducible Protein pS2 also known as Trefoil Factor 1 (TFF1) is a small protein with a mass of about 6.6 kDa. It is mainly expressed in the mucosa of the stomach and other tissues responsive to estrogen. pS2 functions mechanically as part of the trefoil factor family known for its role in mucosal protection and repair. pS2 has a distinctive three-loop structure which is key to its interaction with other molecules and substrates in the cellular environment.

Biological function summary

This trefoil factor plays a significant role in the process of epithelial regeneration and maintaining gastrointestinal tract integrity. It is not known to be part of a complex but works through interaction with various growth factors and cytokines. Its expression is regulated by estrogen making pS2 significant in estrogen-responsive tissues. These interactions contribute to maintaining the stability of epithelial lining aiding in cellular migration and wound healing.

Pathways

PS2 influences cellular signaling involved in the gastric mucosal defense mainly interacting with the Epidermal Growth Factor (EGF) pathway. It modulates the pathway's effects on cell proliferation and migration. Its role is linked to the mitigation of tissue damage indicating a connection with other proteins like the EGF receptor contributing to the repair and defense mechanisms of epithelial tissues.

PS2 has gained attention in breast cancer research. Its expression correlates with hormone receptor-positive breast cancer making it a potential biomarker for the disease. Additionally aberrant expression levels of pS2 might relate to gastric cancer where it plays a role in tumor progression and response to therapy. pS2 is closely connected to estrogen receptor proteins which guide its involvement in these cancers influencing prognosis and treatment strategies.

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Product protocols

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Target data

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

See full target information TFF1

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human TFF1 ELISA Kit

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise