Human TFF3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TFF3 with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.1 - 200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Alternative names
ITF, TFI, TFF3, Trefoil factor 3, Intestinal trefoil factor, Polypeptide P1.B, hITF, hP1.B
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Human TFF3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TFF3 with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.1 - 200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110 | Range 100 - 120 % |
Sample type Serum | Average % = 106 | Range 103 - 109 % |
Sample type EDTA Plasma | Average % = 111 | Range 102 - 122 % |
Sample type Urine | Average % = 106 | Range 99 - 111 % |
Sample type Saliva | Average % = 105 | Range 95 - 110 % |
Sample type Heparin Plasma | Average % = 106 | Range 101 - 112 % |
Sample type Citrate plasma | Average % = 102 | Range 100 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human TFF3 ELISA Kit (ab233617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFF3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFF3 with 1.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Trefoil Factor 3 (TFF3) also known as intestinal trefoil factor is a small peptide with a molecular mass around 7 kDa. This protein is characterized by the presence of a trefoil domain which consists of a stable compact structure important for its function. TFF3 has significant expression in the gastrointestinal tract notably in the colon and small intestine where it acts to maintain the integrity of the mucosal barrier. It can also be found in other tissues but its concentrations vary depending on factors like tissue type and health status.
- Biological function summary
TFF3 plays an important role in protecting and repairing epithelial tissues particularly those lining the gastrointestinal tract. This protein promotes epithelial restitution by aiding cell migration and inhibits apoptosis contributing to tissue healing processes. While TFF3 does not form part of a large protein complex it often interacts with mucins specifically mucin 2 to bolster the protective mucus layer which is essential for shielding epithelial cells.
- Pathways
Trefoil Factor 3 is notably involved in pathways associated with mucosal immune response and inflammation. A significant pathway involving TFF3 is the mucosal defense pathway where it works alongside other mucins and proteins like epidermal growth factor (EGF) to mediate regeneration and defense of epithelial tissues after injury. It also engages in the Wnt signaling pathway where it affects cell proliferation and migration critical for maintaining epithelial homeostasis.
- Associated diseases and disorders
TFF3 has links to inflammatory bowel disease (IBD) and colorectal cancer. In IBD its expression levels may be altered affecting the ability of the intestine to protect itself and heal contributing to the disease's progression. In colorectal cancer TFF3 can exhibit upregulated expression linking it to tumor growth and metastasis. Proteins such as beta-catenin in the Wnt signaling pathway which are associated with cell cycle regulation have connections with TFF3 highlighting its role in cancerous transformations.
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5 product images
Sandwich ELISA - Human TFF3 ELISA Kit (ab233617)
Human TFF3 Competitor standard curve comparison.
Standard curve comparison between Human TFF3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human TFF3 ELISA Kit (ab233617)
Example of human TFF3 standard curve in Sample Diluent NS.
The TFF3 standard curve was prepared as described in Section 10.
Sandwich ELISA - Human TFF3 ELISA Kit (ab233617)
Interpolated concentrations of native TFF3 in human serum and plasma samples.
The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Trefoil Factor 3 concentration was determined to be 5.4 ng/mL in serum, 3.5 ng/mL in plasma (citrate), 5.9 ng/ml in plasma (heparin), and 7.1 ng/ml in plasma (EDTA).
Sandwich ELISA - Human TFF3 ELISA Kit (ab233617)
Interpolated concentrations of native TFF3 in human saliva, urine, and cell culture supernatant samples.
The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1: 4,000, urine 1: 800, and HT-29 supernatant 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Trefoil Factor 3 concentration was determined to be 412.4 ng/ml in saliva, 136.9 ng/ml in urine, and 1.6 ng/mL in HT-29 supernatant.
Sandwich ELISA - Human TFF3 ELISA Kit (ab233617)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF3 concentration was determined to be 7.4 ng/mL with a range of 4.8 – 13.1 ng/mL.
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