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AB233617

Human TFF3 ELISA Kit

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Human TFF3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TFF3 with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ITF, TFI, TFF3, Trefoil factor 3, Intestinal trefoil factor, Polypeptide P1.B, hITF, hP1.B

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ELISA - Human TFF3 ELISA Kit (AB233617)
  • ELISA

Supplier Data

ELISA - Human TFF3 ELISA Kit (AB233617)
Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)
  • sELISA

Supplier Data

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF3 concentration was determined to be 7.4 ng/mL with a range of 4.8 – 13.1 ng/mL.

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)
  • sELISA

Supplier Data

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)

Interpolated concentrations of native TFF3 in human serum and plasma samples.

The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Trefoil Factor 3 concentration was determined to be 5.4 ng/mL in serum, 3.5 ng/mL in plasma (citrate), 5.9 ng/ml in plasma (heparin), and 7.1 ng/ml in plasma (EDTA).

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)
  • sELISA

Supplier Data

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)

Interpolated concentrations of native TFF3 in human saliva, urine, and cell culture supernatant samples.

The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 1 : 4,000, urine 1 : 800, and HT-29 supernatant 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Trefoil Factor 3 concentration was determined to be 412.4 ng/ml in saliva, 136.9 ng/ml in urine, and 1.6 ng/mL in HT-29 supernatant.

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)
  • sELISA

Supplier Data

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)

Example of human TFF3 standard curve in Sample Diluent NS.

The TFF3 standard curve was prepared as described in Section 10.

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)
  • sELISA

Unknown

Sandwich ELISA - Human TFF3 ELISA Kit (AB233617)

Human TFF3 Competitor standard curve comparison.

Standard curve comparison between Human TFF3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.3 pg/mL

Range

3.1 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TFF3 ELISA Kit ab233617 is a rapid single-wash 90-min Sandwich ELISA to measure Human TFF3 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 1.3 pg/mL.

How the assay works

Human TFF3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TFF3 ELISA Kit ab233617 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Trefoil Factor 3 antibody [EPR21130-244] - BSA and Azide free (Capture) ab245003
- Anti-Trefoil Factor 3 antibody [EPR21130-240] - BSA and Azide free (Detector) ab245003
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 120 %", "average": "= 110" }, { "sample": "Serum", "range": "103 - 109 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "102 - 122 %", "average": "= 111" }, { "sample": "Urine", "range": "99 - 111 %", "average": "= 106" }, { "sample": "Saliva", "range": "95 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "101 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "100 - 107 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233617-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233617-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233617-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233617-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233617-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233617-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233617-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TFF3 Capture Antibody", "number":"AB233617-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TFF3 Lyophilized Recombinant Protein", "number":"AB233617-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB233617-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TFF3 Detector Antibody", "number":"AB233617-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trefoil Factor 3 (TFF3) also known as intestinal trefoil factor is a small peptide with a molecular mass around 7 kDa. This protein is characterized by the presence of a trefoil domain which consists of a stable compact structure important for its function. TFF3 has significant expression in the gastrointestinal tract notably in the colon and small intestine where it acts to maintain the integrity of the mucosal barrier. It can also be found in other tissues but its concentrations vary depending on factors like tissue type and health status.
Biological function summary

TFF3 plays an important role in protecting and repairing epithelial tissues particularly those lining the gastrointestinal tract. This protein promotes epithelial restitution by aiding cell migration and inhibits apoptosis contributing to tissue healing processes. While TFF3 does not form part of a large protein complex it often interacts with mucins specifically mucin 2 to bolster the protective mucus layer which is essential for shielding epithelial cells.

Pathways

Trefoil Factor 3 is notably involved in pathways associated with mucosal immune response and inflammation. A significant pathway involving TFF3 is the mucosal defense pathway where it works alongside other mucins and proteins like epidermal growth factor (EGF) to mediate regeneration and defense of epithelial tissues after injury. It also engages in the Wnt signaling pathway where it affects cell proliferation and migration critical for maintaining epithelial homeostasis.

TFF3 has links to inflammatory bowel disease (IBD) and colorectal cancer. In IBD its expression levels may be altered affecting the ability of the intestine to protect itself and heal contributing to the disease's progression. In colorectal cancer TFF3 can exhibit upregulated expression linking it to tumor growth and metastasis. Proteins such as beta-catenin in the Wnt signaling pathway which are associated with cell cycle regulation have connections with TFF3 highlighting its role in cancerous transformations.
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Product protocols

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Target data

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
See full target information TFF3
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websiteProtocolBooklet
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Product promise

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