Human TFPI2 ELISA Kit

Product details

The Human TFPI2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213836) is designed for the quantitative measurement of Human TFPI2 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D23-F235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TFPI2 amount of sample captured in plate.

TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.