Human TFPI2 ELISA Kit

Product details

Human TFPI2 ELISA kit (ab275908) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFPI2 protein in human serum, plasma-citrate, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFPI2 with 16 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

TFPI2, Tissue factor pathway inhibitor 2 also known as placental protein 5 (PP5), is a serine protease inhibitor that plays a key role in plasmin-mediated remodeling of the matrix by inhibiting trypsin and plasmin. TFPI2 is involved in matrix homeostasis, fetal development, and preventing tumor metastasis through invasion. Hypermethylation of this protein is associated with many cancer types, including, colorectal, pancreatic, and cervical cancers. The immunogen used to create the antibodies for this kit represents the mature form of the protein. The protein homologs in mouse in rat share little sequence homology, and as such, are not expected to cross-react with this kit.