JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314703

Human TGE ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human TGE ELISA Kit is a Sandwich ELISA for the measurement of Human TGE in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Protein-glutamine gamma-glutamyltransferase E, Transglutaminase E, Transglutaminase-3, TG(E), TGE, TGase E, TGase-3, TGM3

1 Images
Sandwich ELISA - Human TGE ELISA Kit (AB314703)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGE ELISA Kit (AB314703)

Example of standard curve using ab314703. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.16 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human TGE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TGE in serum (human TGE concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human TGE coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "69 - 92 %", "average": "= 77.88" }, { "sample": "Plasma", "range": "68 - 121 %", "average": "= 83.54" }, { "sample": "Cell culture media", "range": "110 - 135 %", "average": "= 125.7" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314703-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human TGE Protein Standard", "number":"AB314703-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB314703-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314703-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314703-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TGE Detection Antibody", "number":"AB314703-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TGE Antibody-coated ELISA Plate", "number":"AB314703-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314703-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314703-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314703-CMP07", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
See full target information TGM3
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com