The Human transforming growth factor beta 1 (TGF-β1) ELISA kit (colorimetric) is a sandwich ELISA designed to quantify TGF-β1.
Human TGF-β1 ELISA kit offers:
- High sensitivity – detects TGF-β1 as low as 18 pg/mL
- Wide dynamic range – quantifies 18–4,000 pg/mL
- Broad sample compatibility – works with serum, plasma, and cell culture supernatants
- Cited in over 85 publications
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. Latency-associated peptide. Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix (PubMed:28117447). Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1 (PubMed:19651619, PubMed:19750484, PubMed:2022183, PubMed:22278742, PubMed:8617200, PubMed:8939931). Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs) (PubMed:19651619, PubMed:19750484, PubMed:22278742). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Transforming growth factor beta-1. Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (PubMed:29109152). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus (PubMed:19651619, PubMed:19750484, PubMed:2022183, PubMed:22278742, PubMed:8617200, PubMed:8939931). TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed:20207738). While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (By similarity). Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity). At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity). At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed:25893292, PubMed:29483653, PubMed:30696809). Positively regulates odontoblastic differentiation in dental papilla cells, via promotion of IPO7-mediated translocation of phosphorylated SMAD2 to the nucleus and subsequent transcription of target genes (By similarity). Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed:25893292, PubMed:30696809).
TGFB, TGFB1, Transforming growth factor beta-1 proprotein
The Human transforming growth factor beta 1 (TGF-β1) ELISA kit (colorimetric) is a sandwich ELISA designed to quantify TGF-β1.
Human TGF-β1 ELISA kit offers:
- High sensitivity – detects TGF-β1 as low as 18 pg/mL
- Wide dynamic range – quantifies 18–4,000 pg/mL
- Broad sample compatibility – works with serum, plasma, and cell culture supernatants
- Cited in over 85 publications
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97.87 | Range 85 - 104 % |
Sample type Serum | Average % = 94.46 | Range 82 - 102 % |
Sample type Plasma | Average % = 95.78 | Range 93 - 103 % |
Human TGF beta 1 ELISA Kit ab100647 is a sandwich ELISA to measure human TGF beta 1 in serum, plasma, and cell culture supernatant with a sensitivity of 18 pg/mL.
How the assay works
This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGFB 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay protocol summary
Human TGF beta 1 ELISA Kit ab100647 protocol summary:
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotinylated antibody. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
Getting the best performance
CROSS REACTIVITY
This ELISA kit shows no cross-reactivity with any of the cytokines tested; ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-α, GRO-β, GRO-γ, I-309, IFN-γ, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1α, MIP-1β, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ2, TGFβ3, VEGF.
Target information
Transforming Growth Factor-beta 1 (TGF-β1) plays a dual role in cancer, acting as a tumor suppressor in early stages by regulating cell growth and as a tumor promoter in advanced stages by enhancing cell migration and metastasis.
How other researchers are using this kit
Human TGF beta 1 ELISA Kit ab100647 has been used to study inflammation in different physio-pathological contexts such as squamous cell carcinoma (1), gastric cancer (2), and osteoarthritis (3).
References: 1-Yi Huang et al. 2023, PMID: 37872157, 2-Shuai Lv et al. 2024, PMID: 38490994, 3-Zhong Wu, et al. 2023, PMID: 37842584
Related and recommended products
Human TGF beta 1 ELISA Kit ab100647 is commonly used to measure IFN gamma as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
-Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
-Human IL-10 ELISA Kit (Interleukin-10) Human IL-10 ELISA Kit (Interleukin-10) ab185986
-Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
Human TGF beta 1 ELISA Kit ab100647 is a sandwich ELISA to measure human TGF Beta 1 (TGF-β1) in serum, plasma, and cell culture supernatant with a sensitivity of 18 pg/mL.
How the assay works
This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGFB 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay specificity
Cross-reactivity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested; ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-?, GRO-?, GRO-?, I-309, IFN-?, IL-1?, IL-1?, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1?, MIP-1?, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1?, TIMP-1, TIMP-2, TNF?, TGF?2, TGF?3, VEGF.
Human TGF beta 1 ELISA Kit ab100647 protocol summary
1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add biotinylated antibody. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Human TGF beta 1 ELISA Kit ab100647
Human TGF beta 1 ELISA Kit ab100647 has been used to study inflammation in different physio-pathological contexts such as squamous cell carcinoma (1), gastric cancer (2), and osteoarthritis (3).
References:
(1)Yi Huang et al. 2023, PMID: 37872157,Related and recommended products
Human TGF beta 1 ELISA Kit ab100647 is commonly used to measure IFN gamma as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
- Human IL-10 ELISA Kit (Interleukin-10) Human IL-10 ELISA Kit (Interleukin-10) ab185986
- Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
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