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AB193715

Human TGF beta R2 ELISA Kit

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(2 Publications)

Human TGF beta R2 ELISA Kit is a Sandwich ELISA for the measurement of Human TGF beta R2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, TGFBR2

2 Images
Sandwich ELISA - Human TGF beta R2 ELISA Kit (AB193715)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGF beta R2 ELISA Kit (AB193715)

Typical Standard Curve in Assay Diluent A using ab193715 TGF beta R2 Human ELISA Kit.

Sandwich ELISA - Human TGF beta R2 ELISA Kit (AB193715)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGF beta R2 ELISA Kit (AB193715)

Typical Standard Curve in Assay Diluent B using ab193715 TGF beta R2 Human ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.5 pg/mL

Range

1.5 - 500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB259360

Anti-TGF beta Receptor II antibody [EPR24349-124]

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Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TGF beta R2 Human ELISA Kit (ab193715) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta R2 in serum, plasma and cell culture supernatant.

This assay employs an antibody specific for Human TGF beta R2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TGF beta R2 present in the samples. The wells are washed and biotinylated anti-Human TGF beta R2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TGF beta R2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 106 %", "average": "= 97.91" }, { "sample": "Plasma", "range": "87 - 103 %", "average": "= 94.61" }, { "sample": "Cell culture media", "range": "99 - 133 %", "average": "= 121.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193715-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193715-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193715-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193715-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193715-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193715-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TGF-beta R2 detection antibody", "number":"AB193715-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human TGF-beta R2 Microplate (12 strips x 8 wells)", "number":"AB193715-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TGF-beta R2 standards (lyophilized)", "number":"AB193715-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193715-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and thus regulates a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.. Isoform 1. Has transforming growth factor beta-activated receptor activity.. Isoform 2. Has transforming growth factor beta-activated receptor activity.. Isoform 3. Binds TGFB1, TGFB2 and TGFB3 in the picomolar affinity range without the participation of additional receptors. Blocks activation of SMAD2 and SMAD3 by TGFB1.
See full target information TGFBR2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27442 PubMed39523401

2024

Expression of individual members of the TGF-β/SMAD signalling pathway in the progression and survival of patients with colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Večurkovská,Marek Stupák,Jana Kaťuchová,Peter Bohuš,Lenka Hostačná,Mária Mareková,Jana Mašlanková

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:395-403 PubMed33857357

2021

LncRNA NORAD regulates scar hypertrophy via miRNA-26a mediating the regulation of TGFβR1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Qi,Yangyang Wu,Haijian Zhang,Yifei Liu
View all publications
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websiteProtocolBooklet
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Product promise

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