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AB272203

Human TGFB3 ELISA Kit

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Human TGFB3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TGFB3 with a sensitivity of 0.038 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Transforming growth factor beta-3 proprotein, TGFB3

4 Images
ELISA - Human TGFB3 ELISA Kit (AB272203)
  • ELISA

Supplier Data

ELISA - Human TGFB3 ELISA Kit (AB272203)
Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)

Interpolated concentrations of spiked TGFB3 in human heart extract and RPMI cell culture media samples.

The concentrations of TGFB3 were measured in duplicates, interpolated from the TGFB3 standard curves and corrected for sample dilution. Undiluted samples are as follows : heart extract 100 μg/mL, 50% RPMI containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)

Representative image of Human TGFB3 ELISA Kit (ab272203)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human TGFB3 Capture Antibody

- 10X Human TGFB3 Detector Antibody

- Human TGFB3 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGFB3 ELISA Kit (AB272203)

Example of human TGFB3 standard curve in Sample Diluent NS.

The TGFB3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.038 ng/mL

Range

0.38 - 24 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TGFB3 ELISA Kit ab272203 is a rapid single-wash 90-min Sandwich ELISA to measure Human TGFB3 in cell culture media, tissue extracts. This SimpleStep sensitivity is 0.038 ng/mL.

How the assay works

Human TGFB3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TGFB3 ELISA Kit ab272203 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TGF beta 3 antibody [EPR21888-209] - BSA and Azide free (Capture) ab272387
- Anti-TGF beta 3 antibody [EPR21888-220] - BSA and Azide free (Detector) ab272387

Transforming growth factor beta-3 is a cytokine encoded by the TGFB3 gene. TGFB3 belongs to a larger family of proteins known as the transforming growth factor beta superfamily, which includes TGFB1 and 2, bone morphogenetic proteins, Growth Differentiation factors, inhibins and activins. TGFB3 is expressed as a proprotein containing a 277- amino acid latency-associated peptide (LAP) that remains non-covalently associated with TGFB3 after cleavage by the Golgi apparatus. Interaction with integrins removes the LAP, thus releasing a 112- amino acid active TGFB3. Active TGFB3 has many functions, including regulation of the embryogenesis and secondary palate development cell differentiation. The standard protein in this product is the active sequence of TGFB3.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "86 - 96 %", "average": "= 91" }, { "sample": "Cell culture media", "range": "89 - 107 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272203-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272203-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272203-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272203-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272203-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB272203-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272203-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272203-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272203-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272203-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TGFB3 Lyophilized Recombinant Protein", "number":"AB272203-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TGFB3 Capture Antibody", "number":"AB272203-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TGFB3 Detector Antibody", "number":"AB272203-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Transforming growth factor beta-3 proprotein : Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.. Latency-associated peptide. Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).. Transforming growth factor beta-3 : Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins : integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
See full target information TGFB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 37:439-458 PubMed38698918

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Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Ke Li,Chenyang Wang,Ying Rao,Rocky S Tuan,Dan Michelle Wang,Dai Fei Elmer Ker
View all publications
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Product promise

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