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AB213837

Human TGFBR3 ELISA Kit

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Human TGFBR3 ELISA Kit is a Sandwich ELISA for the measurement of Human TGFBR3 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGFR-3, Betaglycan, Transforming growth factor beta receptor III, TGF-beta receptor type III, TGFBR3

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Sandwich ELISA - Human TGFBR3 ELISA Kit (AB213837)
  • sELISA

Supplier Data

Sandwich ELISA - Human TGFBR3 ELISA Kit (AB213837)

Human TGFBR3 ELISA Kit (ab213837) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human TGFBR3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213837) is designed for the quantitative measurement of Human TGFBR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBR3 has been pre-coated onto 96-well plates. Standards (NSO, G21-D781) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TGFBR3 amount of sample captured in plate.

Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, non redundant role in TGF beta signaling.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "186 pg/mL", "standardDeviation": "8.74", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1499 pg/mL", "standardDeviation": "133.41", "coefficientOfVariability": "= 8.9" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "5554 pg/mL", "standardDeviation": "294.36", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "182 pg/mL", "standardDeviation": "11.83", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1370 pg/mL", "standardDeviation": "102.75", "coefficientOfVariability": "= 7.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "5882 pg/mL", "standardDeviation": "376.44", "coefficientOfVariability": "= 6.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213837-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213837-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human TGFBR3 antibody", "number":"AB213837-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human TGFBR3 coated Microplate (12 x 8 wells)", "number":"AB213837-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213837-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213837-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213837-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213837-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213837-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human TGFBR3 standard", "number":"AB213837-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213837-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213837-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Cell surface receptor that regulates diverse cellular processes including cell proliferation, differentiation, migration, and apoptosis (PubMed : 12958365, PubMed : 19416857). Initiates BMP, inhibin, and TGF-beta signaling pathways by interacting with different ligands including TGFB1, BMP2, BMP5, BMP7 or GDF5 (PubMed : 18184661). Alternatively, acts as a cell surface coreceptor for BMP ligands, serving to enhance ligand binding by differentially regulating BMPR1A/ALK3 and BMPR1B/ALK6 receptor trafficking (PubMed : 19726563). Promotes epithelial cell adhesion, focal adhesion formation and integrin signaling during epithelial cell spreading on fibronectin (PubMed : 22562249). By interacting with the scaffolding protein beta-arrestin2/ARRB2, regulates migration or actin cytoskeleton and promotes the activation of CDC42 as well as the inhibition of NF-kappa-B (PubMed : 19416857, PubMed : 19325136). In gonadotrope cells, acts as an inhibin A coreceptor and regulates follicle-stimulating hormone (FSH) levels and female fertility (By similarity). Plays a role in the inhibition of directed and random cell migration in epithelial cells by altering the actin cytoskeletal organization (PubMed : 19416857). Participates in epithelial-mesenchymal transformation (EMT) upon binding to BMP2 or TGFB2, by activating the PAR6/SMURF1/RHOA pathway (By similarity).. (Microbial infection) May act as a receptor for human cytomegalovirus in different cell types by interacting with HCMV trimer composed of GO, GH and GL.
See full target information TGFBR3
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Product promise

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