Human THBS2 ELISA Kit
- Recombinant
- SimpleStep
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Human THBS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human THBS2 with a sensitivity of 0.024 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
TSP2, THBS2, Thrombospondin-2
- sELISA
Supplier Data
Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)
Human THBS2 standard curve comparison.
Standard Curve comparison between human THBS2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)
Interpolated concentrations of native THBS2 in human serum and plasma samples.
The concentrations of THBS2 were measured in duplicates, interpolated from the THBS2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 25%, plasma (EDTA) 50%, and plasma (CTAD) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 17.55 ng/mL in neat serum, 8.328 ng/mL in neat plasma (citrate), 18.15 ng/mL in neat plasma (heparin), 11.22 ng/mL in neat plasma (EDTA), and 8.524 ng/mL in neat plasma (CTAD).
- sELISA
Supplier Data
Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)
Serum from nine individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 17.83 ng/mL with a range of 12.01 – 21.94 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)
Interpolated concentrations of native THBS2 in human breast milk samples.
The concentrations of THBS2 were measured in duplicates, interpolated from the THBS2 standard curves and corrected for sample dilution. Undiluted samples are as follows : breast milk 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 0.825 ng/mL in neat human breast milk (defatted).
- sELISA
Supplier Data
Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)
Example of human THBS2 standard curve in Sample Diluent 75BS.
The THBS2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human THBS2 ELISA Kit (ab260051) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of THBS2 protein in cit plasma, edta plasma, hep plasma, milk, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human THBS2 with 0.024 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
THBS2 (Thrombospondin-2) is a secreted cell adhesion glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It is a ligand for CD36 which mediates antiangiogenic properties.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TSP2 modulates angiogenesis the process of new blood vessel formation. It functions as an inhibitor of angiogenesis contributing to the regulation of tissue repair and remodeling. TSP2 does not operate alone; it is part of a larger complex within the extracellular matrix that also involves other thrombospondins like Thrombospondin 1 with which it shares some functional similarities. These thrombospondins collaborate to influence cell adhesion and migration.
Pathways
TSP2 participates significantly in the TGF-β signaling pathway and the regulation of vascular endothelial growth factor (VEGF) activity. Through the TGF-β pathway TSP2 influences extracellular matrix remodeling and tissue fibrosis. Additionally its interaction with VEGF modulates angiogenesis where TSP2 acts as an antagonist counterbalancing the effects of VEGF. This interaction links TSP2 with regulatory mechanisms involving proteins like integrins which play roles in the cellular attachment to the matrix.
Product protocols
- Visit the General protocols
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Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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