Human THBS4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human THBS4 ELISA Kit in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.1 - 25 ng/mL
- Sensitivity
- = 0.1 ng/mL
Target data
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner (By similarity).
Alternative names
TSP4, THBS4, Thrombospondin-4
What's included?
Recommended products
Human THBS4 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human THBS4 ELISA Kit in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.1 - 25 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.1 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 87.68 | Range 77 - 96 % |
Sample type Plasma | Average % = 83.57 | Range 69 - 98 % |
Sample type Cell culture media | Average % = 73.34 | Range 69 - 78 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human THBS4 (Thrombospondin-4) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human THBS4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human THBS4 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
THBS4 also known as Thrombospondin-4 is a glycoprotein with a mass of approximately 105 kDa. It is part of the thrombospondin family which is known for binding to various cell surface receptors and components of the extracellular matrix. THBS4 is expressed in many tissues including the brain heart and skeletal muscle. Its structure allows it to interact with other proteins and play a role in cell-matrix communication.
- Biological function summary
THBS4 interacts with molecules in the extracellular matrix to regulate cellular functions such as adhesion migration and proliferation. It often operates within a complex network of proteins modulating cell interactions and tissue remodeling. This protein contributes to synaptic functions in the nervous system and soft tissue repair. The presence of THBS4 in different tissue types suggests it has diverse roles across the body's systems.
- Pathways
This protein is a component of the signaling pathways related to inflammatory and repair processes. THBS4 participates in the TGF-beta (Transforming Growth Factor-beta) signaling pathway which involves other proteins like TGF-beta itself and integrins. It influences wound healing and fibrosis through its effects on cellular behavior and signaling cascades.
- Associated diseases and disorders
Alterations in THBS4 levels can associate with conditions such as myocardial infarction and osteoarthritis. Abnormal expression or function of THBS4 can disrupt normal tissue repair and inflammation control contributing to disease progression. The protein's interaction with integrins and collagen is significant in understanding its role in these conditions highlighting its potential as a therapeutic target for modulating tissue repair and disease processes.
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1 product image
Sandwich ELISA - Human THBS4 ELISA Kit (ab314715)
Example of standard curve using ab314715. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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