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AB277448

Human Thioredoxin 1 ELISA Kit

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Human Thioredoxin 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Thioredoxin 1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

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ELISA - Human Thioredoxin 1 ELISA Kit (AB277448)
  • ELISA

Supplier Data

ELISA - Human Thioredoxin 1 ELISA Kit (AB277448)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Thioredoxin 1 ELISA Kit (ab277448) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thioredoxin 1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Thioredoxin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Thioredoxin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Thioredoxin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Thioredoxin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 106 %", "average": "= 98.98" }, { "sample": "Plasma", "range": "87 - 104 %", "average": "= 93.86" }, { "sample": "Cell culture media", "range": "100 - 115 %", "average": "= 108.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277448-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277448-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"1200X HRP-Streptavidin Concentrate", "number":"AB277448-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277448-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Thioredoxin 1 coated Microplate", "number":"AB277448-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277448-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277448-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thioredoxin 1 standard protein (Lyophilized)", "number":"AB277448-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Thioredoxin 1 Antibody", "number":"AB277448-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin 1 also known as TRX1 or TXN is a small redox protein with an approximate mass of 12 kDa. Mechanically it functions as a thiol-disulfide oxidoreductase facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin 1 is ubiquitously expressed found in the cytoplasm and also in the nucleus in response to certain signals. Its expression levels can change depending on cellular conditions highlighting its adaptive role in response to oxidative stress.
Biological function summary

Thioredoxin 1 acts as a central player in cellular processes by maintaining a reduced environment in the cell. It serves as an antioxidant modulating the redox state of proteins and is part of the broader thioredoxin system which includes thioredoxin reductase and NADPH. By promoting the reduction of oxidative species and repairing oxidatively damaged proteins it supports many cellular functions such as cell proliferation and apoptosis. This makes it an important part of the intracellular redox balance.

Pathways

Thioredoxin 1 functions within important signaling cascades including the MAPK and NF-kB pathways. In the MAPK pathway it regulates signal transduction by influencing the phosphorylation status of key proteins while in the NF-kB pathway it affects the activation of transcription factors related to inflammation and immune response. The interaction with these pathways links Thioredoxin 1 to other proteins such as ASK1 and IKK contributing to stress response and inflammation.

Thioredoxin 1 has been implicated in cancer and cardiovascular disease. Its overexpression can lead to persistent reduction states favorable for tumor growth and survival and it's often observed in various cancer types. In cardiovascular disease the dysregulation of its activity contributes to oxidative stress influencing conditions such as atherosclerosis. Through these pathways it connects with proteins like VEGF in cancer and Nitric Oxide Synthase in cardiovascular diseases indicating its diverse role in pathological processes.
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Product protocols

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Target data

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
See full target information TXN
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