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AB108907

Human Thrombin-Antithrombin Complex ELISA Kit (TAT)

4

(3 Reviews)

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(24 Publications)

Human Thrombin-Antithrombin Complex ELISA Kit (TAT) is a sandwich ELISA designed to quantify Human Thrombin-Antithrombin Complex (TAT) with a sensitivity of 0.86 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.48 - 120 ng/mL
- Cited in over 10 publications
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Sandwich ELISA - Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (AB108907)
  • sELISA

Supplier Data

Sandwich ELISA - Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (AB108907)

Representative Standard Curve Using ab108907.

Sandwich ELISA - Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (AB108907)
  • sELISA

Lab

Sandwich ELISA - Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (AB108907)

Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit.

TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Milk, Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.86 ng/mL

Range

1.48 - 120 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Human Thrombin-Antithrombin Complex ELISA Kit (TAT) ab108907 is a sandwich ELISA to measure Human Thrombin-Antithrombin Complex (TAT) in plasma, milk with a sensitivity of 0.86 ng/ml.

How the assay works

A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Thrombin-Antithrombin Complex ELISA Kit (TAT) ab108907 protocol summary

1. Add standards or samples to appropriate wells and incubate for 2 hours
2. wash
3.Add Biotinylated Thrombin-Antithrombin Complex Antibody to each well and incubate for 1 hour
4.Wash
SP Conjugate to each well and incubate for 20 minutes
5. Wash
6. Add Chromogen Substrate to each well and incubate for 20 min
7. Add stop soution and read at 450 nm.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thrombin-Antithrombin Complex also referred to as TAT complex plays a significant mechanical role in regulating blood coagulation. It forms from the interaction of thrombin an enzyme and antithrombin a serpin inhibitor. Thrombin shows an approximate molecular mass of 36 kDa while antithrombin weighs around 58 kDa. This complex is expressed in the liver and circulates in the bloodstream where it helps maintain hemostasis by neutralizing excess thrombin preventing unwanted clotting events.
Biological function summary

The complex participates in the regulation of the coagulation cascade. It functions as an essential inhibitor forming part of a larger set of interactions that ensure blood coagulation occurs correctly. As a result it plays a role in maintaining the balance between coagulation and anticoagulation reducing the risk of thrombotic conditions. The TAT complex can be detected using specific kits like TAT kits which measure its levels important for understanding coagulation status in conditions using both human and mouse models.

Pathways

The thrombin-antithrombin complex is integrally involved in the intrinsic and extrinsic pathways of the coagulation cascade. It serves as a feedback mechanism to control and limit thrombin activity. The balance it provides is important to the effective regulation of fibrin formation working alongside proteins such as fibrinogen and factor Xa. Antithrombin in particular requires heparin or heparin-like molecules to enhance its inhibitory effects on thrombin ensuring precise modulation of coagulation.

Thrombin-antithrombin complex levels correlate with various thrombotic and hemorrhagic disorders. Elevated levels often indicate ongoing activation of the coagulation system seen in conditions like deep vein thrombosis and disseminated intravascular coagulation (DIC). These elevated levels can result from increased thrombin generation and antithrombin consumption. The complex's interaction with proteins like factor V and fibrin is pertinent in the pathology of these disorders making it a valuable marker in diagnosis and monitoring therapeutic responses.
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Product protocols

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Target data

See full target information Thrombin-Antithrombin Complex
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Pulmonary circulation 14:e12454 PubMed39386377

2024

Didang decoction attenuates cancer-associated thrombosis by inhibiting PAD4-dependent NET formation in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zeng,Jiuxi Li,Liyuan Pei,Yaping Yang,Ya Chen,Xuejing Wang,Ting Zhang,Ting Zhou

Journal of neuroinflammation 21:237 PubMed39334416

2024

Cerebrospinal fluid markers of neuroinflammation and coagulation in severe cerebral edema and chronic hydrocephalus after subarachnoid hemorrhage: a prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjian Fang,Yibo Liu,Luxi Chen,Junjie Wang,Jiahao Zhang,Haocheng Zhang,Sixuan Tian,Anke Zhang,Jianmin Zhang,John H Zhang,Xiaoyu Wang,Jun Yu,Sheng Chen

Nature 622:393-401 PubMed37821590

2023

Design and testing of a humanized porcine donor for xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ranjith P Anand,Jacob V Layer,David Heja,Takayuki Hirose,Grace Lassiter,Daniel J Firl,Violette B Paragas,Adam Akkad,Sagar Chhangawala,Robert B Colvin,Russell J Ernst,Nicholas Esch,Kristen Getchell,Alexandra K Griffin,Xiaoyun Guo,Katherine C Hall,Paula Hamilton,Lokesh A Kalekar,Yinan Kan,Ahmad Karadagi,Feng Li,Susan C Low,Rudy Matheson,Claudia Nehring,Ryo Otsuka,Matthew Pandelakis,Robert A Policastro,Rebecca Pols,Luis Queiroz,Ivy A Rosales,William T Serkin,Kathryn Stiede,Toshihide Tomosugi,Yongqiang Xue,Gabriel E Zentner,David Angeles-Albores,J Chris Chao,Juliet N Crabtree,Sierra Harken,Nicole Hinkle,Tania Lemos,Mailin Li,Lorena Pantano,Denise Stevens,Omar D Subedar,Xiaoqing Tan,Shiyi Yin,Imran J Anwar,David Aufhauser,Saverio Capuano,Dixon B Kaufman,Stuart J Knechtle,Jean Kwun,Dhanansayan Shanmuganayagam,James F Markmann,George M Church,Mike Curtis,Tatsuo Kawai,Michele E Youd,Wenning Qin

Journal of innate immunity 15:412-427 PubMed36858027

2023

Combined Heterozygous Genetic Variations in Complement C2 and C8B: An Explanation for Multidimensional Immune Imbalance?

Applications

Unspecified application

Species

Unspecified reactive species

Marco Mannes,Rebecca Halbgebauer,Lisa Wohlgemuth,David Alexander Christian Messerer,Susa Savukoski,Anke Schultze,Bettina Berger,Christiane Leonie Knapp,Christoph Q Schmidt,Daniel Fürst,Morten Hillmer,Reiner Siebert,Oskar Eriksson,Barbro Persson,Bo Nilsson,Kristina Nilsson Ekdahl,Markus Huber-Lang

Cellular and molecular gastroenterology and hepatology 14:1077-1101 PubMed35926777

2022

FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xitang Li,Qiang Gao,Wenhui Wu,Suping Hai,Junjian Hu,Jie You,Da Huang,Hongwu Wang,Di Wu,Meifang Han,Dong Xi,Weiming Yan,Tao Chen,Xiaoping Luo,Qin Ning,Xiaojing Wang

Frontiers in cardiovascular medicine 9:887664 PubMed35935621

2022

Contact pathway in surgical and transcatheter aortic valve replacement.

Applications

Unspecified application

Species

Unspecified reactive species

María Eugenia de la Morena-Barrio,Javier Corral,Cecilia López-García,Víctor Alonso Jiménez-Díaz,Antonia Miñano,Pablo Juan-Salvadores,María Asunción Esteve-Pastor,José Antonio Baz-Alonso,Ana María Rubio,Francisco Sarabia-Tirado,Miguel García-Navarro,Juan García-Lara,Francisco Marín,Vicente Vicente,Eduardo Pinar,Sergio José Cánovas,Gonzalo de la Morena

JAMA surgery 157:713-721 PubMed35731524

2022

Association of Changes in Antithrombin Activity Over Time With Responsiveness to Enoxaparin Prophylaxis and Risk of Trauma-Related Venous Thromboembolism.

Applications

Unspecified application

Species

Unspecified reactive species

Laura E Vincent,Michael M Talanker,Dakota D Butler,Xu Zhang,Jeanette M Podbielski,Yao-Wei W Wang,Amber Chen-Goodspeed,Selina L Hernandez Gonzalez,Erin E Fox,Bryan A Cotton,Charles E Wade,Jessica C Cardenas

Scientific reports 11:21852 PubMed34750441

2021

Human endothelial cells and fibroblasts express and produce the coagulation proteins necessary for thrombin generation.

Applications

Unspecified application

Species

Unspecified reactive species

Clay T Cohen,Nancy A Turner,Joel L Moake

Intensive care medicine experimental 9:41 PubMed34396466

2021

Complement factor D is linked to platelet activation in human and rodent sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

O Sommerfeld,K Dahlke,M Sossdorf,R A Claus,A Scherag,M Bauer,F Bloos

Frontiers in cellular and infection microbiology 11:677902 PubMed34336711

2021

Tissue Factor-Enriched Neutrophil Extracellular Traps Promote Immunothrombosis and Disease Progression in Sepsis-Induced Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Yilu Zhou,Mengdi Qu,Ying Yu,Zhaoyuan Chen,Shuainan Zhu,Kefang Guo,Wankun Chen,Changhong Miao
View all publications
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