Human Thrombin Antithrombin Complex total antigen assay ELISA kit is a Quantitative ELISA kit for the measurement of Human Thrombin Antithrombin Complex total antigen assay in Human in Citrate plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum samples.
Colorimetric
Citrate plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum
Quantitative
Human
1 - 1000 ng/mL
= 0.718 ng/mL
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SERPINC1
Human Thrombin Antithrombin Complex total antigen assay ELISA kit is a Quantitative ELISA kit for the measurement of Human Thrombin Antithrombin Complex total antigen assay in Human in Citrate plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum samples.
Colorimetric
Citrate plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum
Quantitative
Human
1 - 1000 ng/mL
Pre-coated microplate (12 x 8 well strips)
= 0.718 ng/mL
Blue Ice
+4°C
+4°C
+4°C
Human Thrombin Antithrombin (TAT) Complex total antigen assay ELISA kit is intended for the quantitative determination of the covalent complex of thrombin and its inhibitor antithrombin III in human plasma and serum.
Human TAT Complex will bind to the affinity purified Anti-Human Thrombin capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal Anti‑Human Antithrombin primary antibody binds to the TAT Complex captured on the plate. Excess antibody is washed away and bound monoclonal antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human TAT complex. Color development is proportional to the concentration of TAT complex in the samples.
Free Thrombin, Prothrombin and Antithrombin III will not be detected by this assay.
This supplementary information is collated from multiple sources and compiled automatically.
Thrombin/Antithrombin complex also known as TAT complex forms when thrombin binds to antithrombin during the process of blood coagulation. Thrombin is an enzyme with a molecular mass of approximately 37 kDa and serves as a critical serine protease in the coagulation cascade. Antithrombin weighing roughly 58 kDa acts as a serine protease inhibitor. The complex primarily forms in the liver and circulates in the bloodstream. Thrombin cleaves fibrinogen to fibrin leading to blood clot formation while antithrombin inhibits thrombin's proteolytic activity therefore regulating coagulation.
The interaction between thrombin and antithrombin is an important mechanism in controlling the clotting process. The thrombin/antithrombin interaction produces the TAT complex effectively neutralizing thrombin’s activity preventing excessive clot formation. This regulation is essential for maintaining hemostatic balance therefore avoiding uncontrolled bleeding or thrombosis. The balance keeps the coagulation system functioning properly by temporarily halting thrombin activity.
Thrombin/antithrombin complex is central to the coagulation cascade and the regulation of fibrinolysis. The coagulation pathway involves the conversion of prothrombin to thrombin which then processes fibrinogen to an active fibrin clot. The fibrinolytic pathway involves plasminogen activation leading to clot dissolution. Antithrombin also interacts with other key proteins like factor Xa reinforcing the inhibitory control over thrombin and associated clotting factors.
Abnormalities in thrombin/antithrombin regulation can lead to conditions such as thrombosis and disseminated intravascular coagulation (DIC). Thrombosis occurs when inappropriate clotting blocks blood vessels while DIC involves widespread clotting and subsequent bleeding. The imbalance between thrombin production and antithrombin inhibition often involves proteins like factor V and protein C which can exacerbate or mitigate these conditions. Understanding this complex is critical for developing therapeutic approaches to prevent and treat coagulation disorders.
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Linear range exaple data.
A typical standard curve for Human TAT complex (linear range). Example only.
Full range example data.
A typical standard curve for Human TAT complex (full range). Example only.
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